Jue Ou Yang

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OBJECTIVE To clone and express 3'-terminal of Parkin gene in E. coli, and prepare its antiserum for further study. METHODS The glutathion-sulfate-transferase (GST) fusion expression plasmid of 3'-terminal of Parkin gene (937-1959 bp) was constructed and transferred to JM 105. After being treated with Triton-100 (1%) and Tween-20 (1%) and purified with(More)
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