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The quality of frozen minced red hake was assessed by FTNIR spectroscopy in transmission mode. The region 1530-1866 nm was best correlated to dimethylamine (DMA) concentration, which is an accepted quality index for frozen gadoid fish. Partial least-squares (PLS) analysis showed that this technique predicts the DMA content sufficiently well for quality(More)
Confocal Raman Microspectroscopy (CRM) can be used as a tool for the in situ evaluation of the chemical composition of living, fully submerged, unstained biofilms. In this study the estimation of the local water content in Pseudomonas aeruginosa PA01 biofilms is given as an example. The ratio of the area of the O-H stretching vibration band at 3450 cm(-1),(More)
AIMS To investigate the use of confocal Raman microspectroscopy (CRM) for the analysis of the structure, composition and development of fully hydrated biofilms. METHODS AND RESULTS Pseudomonas aeruginosa PAO1 biofilms were cultured in a flow cell in minimal nutrient medium (artificial sea water) and their development was followed for up to 3 weeks. The(More)
The distribution of a carotene-containing yeast (CCY) in a biofilm formed by a small colony variant (SCV) of Pseudomonas aeruginosa PA01 was followed by confocal Raman microspectroscopy (CRM). SCV PA01 and CCY cells were distinguished by their spectral signatures, and the distribution of the overall biomass was monitored by the C-H bending or stretching(More)
The growth of Pseudomonas aeruginosa PAO1 biofilms on ZnSe internal reflection elements (IREs) was compared with their growth on TiO(2)-coated ZnSe over several days using attenuated total reflection Fourier transform infrared (ATR-FT-IR) spectroscopy. The effect of the TiO(2) coating on the IR spectra of reference compounds and cell suspensions was(More)
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