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Iminobiotin affinity columns and their application to retrieval of streptavidin.
A method is described for the retrieval of streptavidin from the culture broth of Streptomyces avidinii. The key step in this procedure is the adsorption of streptavidin from culture concentrates toExpand
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Avidin binding of carboxyl-substituted biotin and analogues.
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Purification and characterization of the 110-kDa actin- and calmodulin-binding protein from intestinal brush border: a myosin-like ATPase.
Publisher Summary This chapter describes a new method for purifying the 110-kDa protein calmodulin complex to homogeneity in milligram quantities from chicken intestinal epithelial cells. ThisExpand
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Brush border myosin heavy chain phosphorylation is regulated by calcium and calmodulin
Myosin from chicken intestinal brush borders is phosphorylated on its heavy chains at threonine by a kinase isolated from brush borders. In contrast to other heavy chain kinases, the brush borderExpand
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ACTH antagonists.
Structural modifications within the active site of the ACTH molecule have produced analogs that inhibit the hormone sensitive adenylate cyclase system of bovine adrenal cortical plasma membranes. ItExpand
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Studies with the S-peptide-S-protein system: The role of glutamic acid-2, lysine-7, and methionine-13 in S-peptide1–14 for binding to and activation of S-protein
Abstract Syntheses are described of 12-serine S-peptide 1–14 , 12-(β-pyrazolyl-3)-alanine S-peptide 2–14 , 12-(β-pyrazolyl-3)-alanine S-peptide 3–14 , 13-norleucine S-peptide 1–14 , 13-norleucineExpand
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Hormone-receptor studies with avidin and biotinylinsulin-avidin complexes.
Avidin can be labeled to high specific radioactivity by introducing 3-(p-hydroxyphenyl)-propionyl groups into the molecule (pHPP-avidin). 125I-pHPP-avidin binds avidly to rat liver plasma membranesExpand
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Adenylate cyclase system of bovine adrenal plasma membranes.
The adenylate cyclase system present in a preparation enriched in plasma membranes derived from bovine adrenal cortex was investigated in considerable detail. This system is stimulated byExpand
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Phosphorylation of brush border myosin at threonine on its 20 kDa light chains by a calmodulin‐independent kinase activates its ATPase
A calmodulin‐independent kinase isolated from chicken intestinal brush border phosphorylates brush border myosin mainly at an apparently single threonine on its 20 kDa light chains. PhosphorylationExpand