Juana Sellés

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The mechanism of action of progesterone (Pg) on rat vascular tissue was investigated. We obtained evidence that 10-nM Pg inhibited platelet aggregation at 1-5 min. Previously, we reported that nitric oxide (NO) mediated this antiaggregatory effect. Rat aortic strips (RAS) NO synthase (NOS) activity in response to "in vitro" treatment with other sex steroids(More)
Pollen extracts from Atriplex latifolia, Beta vulgaris, Salsola kali and Amaranthus retroflexus were compared with an extract from Chenopodium album by both in vivo and in vitro methods. Skin prick tests on 20 C. album-sensitive patients were positive with all extracts. RAST inhibition together with two-dimensional immunoelectrophoresis and two-dimensional(More)
1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] produces an acute stimulation of calcium influx in cultured chick embryo myocytes through activation of voltage-gated Ca2+ channels and involvement of cyclic AMP-dependent protein kinase A (PKA). To investigate the participation of protein kinase C (PKC) in this hormone-induced response, calcium uptake was measured in(More)
Several studies have suggested that vitamin D plays a role in cardiovascular function. It has been recently shown that in vitro treatment of vitamin D-deficient chick cardiac muscle with physiological concentrations of 1,25-dihydroxy-vitamin D3 (1,25(OH)2D3) induces a rapid (1-10 min) increase of tissue 45Ca uptake which can be suppressed by Ca channel(More)
The fast actions of the secosteroid hormone 1alpha,25-dihydroxyvitamin D3 [1,25(OH)2D3; calcitriol] and the synthetic analogues calcipotriol (MC 903) and 20-epi-1alpha,25(OH)2D3 (MC 1288) on cell calcium influx were compared in rat duodenum enterocytes as well as in cells from chick embryo skeletal muscle (myoblasts) and heart (myocytes), at various(More)
1,25-Dihydroxyvitamin D3 has been shown to induce rapid changes in calcium fluxes in skeletal muscle and other target tissues independently of gene activation. The possibility that the hormone would produce similar effects in heart where 1,25-dihydroxyvitamin D3 receptors and activities have been shown, was studied. A significant increase of 45Ca uptake by(More)
The rapid non-genomic stimulatory action of progesterone (Pg) and estradiol (E2) on nitric oxide synthase (NOS) activity of endothelium intact aortic rings and its effect on platelet aggregation was investigated. First we measured the effect of the hormones on platelet aggregation when added to rat aortic strips (RAS) incubated in a PRP. RAS induced an(More)
Recent studies have provided evidence indicating that 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] rapidly stimulates calcium influx through Ca2+ channels in isolated chick heart tissue and cells. Studies were performed both to evaluate the characteristics of the hormone action in cultured myocytes and to obtain information on the metabolic pathway which mediates(More)
UNLABELLED Argentinean women have one of the highest international mortality rates for cardiovascular disease and they are particularly vulnerable to eating pathologies. Cardiovascular risk is exacerbated in women with Anorexia Nervosa (AN), since high cholesterol concentrations have been widely reported. OBJECTIVES To compare blood cholesterol(More)
1. The ability of synthetic analogues of the secosteroid hormone 1alpha,25-dihydroxy-vitamin-D3 [calcitriol, CT; 1,25(OH)2D3] to exert non-genomic (rapid) effects on target cells has been scarcely studied. To evaluate the pharmacological potential of the CT side-chain analogues CB1093 and GS1500, we compared their fast effects on intracellular calcium(More)