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Comparative studies of carnation micropropagation under four different ventilation rates showed that using gas-permeable filters, with gelled or liquid media and modifying the volume of culture medium, it was possible to establish a suitable hydric state to obtain good proliferation rates with gelled and liquid medium, as well as optimal acclimatization of(More)
The response to drought of 20 open-pollinated families from four Pinus pinaster Ait. populations covering a latitudinal cline (France, Central and Southern Spain, and Morocco) was assessed. The seedlings were cultivated in a greenhouse under controlled temperature and vapor pressure deficit for 120 days, and subjected to two watering regimes for 60 days.(More)
Vessel closure configurations exert direct and indirect control over factors pertaining to the physical boundaries of the mieroenvironment, and induce a typical phenotype in in vitro plant production. Upon modification of the in vitro environment, carnation explants showed a gradation of their ultrastructural characteristics from hyperhydric to normal. A(More)
Establishment of microplants is related to the moisture vapourtransmission of the culture vessel lid. In this respect, stomatal andcuticular physiology were characterized in detached leaves from Dianthuscaryophyllus grown in the glasshouse or in vitro at different rates ofventilation. In vitro plants grown in non-ventilated culture vessels hadless waxes and(More)
We compared the performance of mycorrhiza-inoculated and non-inoculated radiata pine (Pinus radiata D. Don) seedlings in two field studies. In the first study, above ground growth of 2-year-old container-grown trees was compared after planting in two areas that differed in water availability. Mycorrhizal inoculation significantly improved above ground(More)
The role of ethylene in the organogenesis in vitro of nodal segments of poplar (Populus tremula L) was studied. The results indicate that there is a relationship between organogenesis and ethylene because inhibition of its synthesis with AVG, added to the standard MS medium containing naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP), inhibited(More)
Growth and propagation rates, hyperhydricity percentages, macronutrientabsorption and pH evolution were evaluated in Dianthuscaryophyllus CV Nelken cultured in vitro under different ventilationconditions. Culture in well ventilated conditions (HVC) i.e. low relativehumidity, generated lower percentages of hyperhydric explants, with highermicropropagation(More)
We have developed a high-yielding procedure for the in vitro propagation of juvenile material of Taxus baccata involving a combination of seed handling and culture on WP culture medium supplemented with sucrose (2%), activated charcoal (0.5%) and BAP (22.19 mM) for 30 days, followed by 40 days on hormone-free medium. Shoot apical ends should be decapitated(More)
• Interpopulation variation in key functional traits of Pinus pinaster Ait. is well recognized. However, the relative importance of drought tolerance to explain this regional variation in the species remains elusive. • Here, we raise the question whether water availability constitutes a likely driver of regional variation in biomass allocation, growth and(More)
The leaf surfaces of Dianthus caryophyllus plants cultured in vitro in either airtight or ventilated vessels were examined using scanning electron microscopy (SEM). The resultant hyperhydrated, non-hyperhydrated and acclimatized plants were compared for stomatal density, cuticular wax development and stomatal function. The leaf surfaces of in vitro cultured(More)