Juan Lopez

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A link between Tau phosphorylation and aggregation has been shown in different models for Alzheimer disease, including yeast. We used human Tau purified from yeast models to generate new monoclonal antibodies, of which three were further characterized. The first antibody, ADx201, binds the Tau proline-rich region independently of the phosphorylation status,(More)
Ontologies define hierarchies of classes and attributes; they are meta-data: data about data. In the " traditional approach " to on-tology engineering, experts add new data by carefully analyzing others' ontologies and fitting their new concepts into the existing hierarchy. In the emerging " Semantic Web approach " , ordinary users may not look at anyone's(More)
Ontologies define hierarchies of classes and attributes; they are meta-data: data about data. XML Schema and RDF Schema are both (lightweight) ontology definition languages in that sense. In the " traditional " approach to ontology engineering, experts add new data by carefully analyzing others' ontologies and fitting their new concepts into the existing(More)
A cost-effective b[combining low line]a[combining low line]ttery-powered s[combining low line]pectrophotometric s[combining low line]ystem (BASS) was developed for quantitative point-of-care (POC) analysis on a microfluidic chip. By using methylene blue as a model analyte, we first compared the performance of the BASS with a commercial spectrophotometric(More)
The conformational state of distinct prolines can determine the folding of a protein but equally other biological processes when coupled to a conformation-sensitive secondary reaction. For the neuronal tau protein, the importance of proline conformation is underscored by its interaction with different prolyl cis/trans isomerases. The proline conformation(More)
We describe a new efficient strategy for the sequential assignment of amide resonances of a conventional (15)N-(1)H HSQC spectrum of intrinsically unfolded proteins, based on composite NOESY-TOCSY and TOCSY-NOESY mixing times. These composite mixing times lead to a Hα-proton mediated unidirectional transfer of amide to amide proton. We have implemented the(More)