Juan Antonio Sáez-Nieto

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OBJECTIVES A large clonal outbreak of multidrug-resistant CC17 ST17 Enterococcus faecium containing Tn5382 in a hospital in the north of Spain is described. METHODS We characterized vancomycin-resistant E. faecium isolates from 10 infected and 40 colonized inpatients from a single hospital by PFGE, multiple-locus variable-number tandem-repeat analysis(More)
OBJECTIVES To ascertain the participation of topoisomerase mutations in the development of ciprofloxacin resistance in isogenic Stenotrophomonas maltophilia mutants. METHODS gyrAB and parCE sequences in three paired in vivo isogenic ciprofloxacin-susceptible (MIC range 0.5-4 mg/L) and resistant (16-128 mg/L) S. maltophilia strains (PFGE-characterized)(More)
T O T H E EDITOR—Burkholderia cepacia has been linked to nosocomial outbreaks caused by contamination of medical devices, 1 parenteral and nebulized medication, 2 " 4 antiseptic solutions, and other environmental sources. 5 ' 6 It has also been implicated in 2 nosocomial outbreaks caused by contamination of alcohol-free mouthwash. 7 ' 8 Nevertheless,(More)
tion of peste des petits ruminants virus and prospects for improved diagnosis and control.dence of peste des petits ruminants (PPR) virus circulation in Algeria (Sahrawi territories): outbreak investigation and virus lineage identification. To the Editor: The genus Exigu-obacterium was first described in 1983 by Collins et al., who characterized the species(More)
The soil-borne pathogen Nocardia sp. causes severe cutaneous, pulmonary, and central nervous system infections. Against them, co-trimoxazole (SXT) constitutes the mainstay of antimicrobial therapy. However, some Nocardia strains show resistance to SXT, but the underlying genetic basis is unknown. We investigated the presence of genetic resistance(More)
Nocardia species are difficult to identify, a consequence of the ever increasing number of species known and their homogeneous genetic characteristics. 16S rRNA analysis has been the gold standard for identifying these organisms, but proteomic techniques such as matrix-assisted laser desorption ionization-time of flight (MALDI-TOF MS) and housekeeping gene(More)
BACKGROUND We describe a case of traumatic ocular endophthalmitis caused by Nocardia kruczakiae after vegetable trauma in an immunocompetent child. FINDINGS A 5-year-old boy suffered from a trauma with a palm tree leaflet. Two months later, he was diagnosed with traumatic infectious uveitis and intumescent cataract with anterior capsule rupture. Intensive(More)
Nosocomial outbreaks of multidrug-resistant Acinetobacter baumannii are of worldwide concern. Using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and multiple locus variable number tandem repeat sequence (VNTR) analysis (MLVA), the present work examines the genetic diversity of the endemic and epidemic A. baumannii clones(More)
HM. Prevalence of mycoplasmas in eggs from birds of prey using culture and a genus-specifi c mycoplasma polymerase chain reaction. Mycoplasma lipofaciens, a new species of avian origin. Pathogenicity of M. lipofaciens strain ML64, isolated from an egg of a Northern Goshawk (Accipiter gentilis), for chicken embryos. Bradbury JM. Investigations into the(More)
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