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By second-step mutagenesis and treatment with N-methyl-N’-nitro-N-nitrosoguanidine a mutant strain ofMycobacterium sp. V-649 producing a glucan extracellular polymer and another new streptomycin-resistant mutant were prepared. This mutant strain formed more than 100% first-rate (1.0–1.2%) exocellular polysaccharide. Treatment with 1% dimethyl sulfoxide(More)
Agrobacterium sp. and related species which in the soil and in the rhizosphere of wheat accompany the fungusGaeumannomyces graminis var.tritici and cause take-all of the wheat roots produced polysaccharides in pure cultures (glucans, mannoglucans and galactomannoglucans). These polysaccharides were utilized better by the mycelium ofO. graminis than glucose(More)
Production of the exocellular polysaccharide of the phytopathogenic bacteriumXanthomonas fuscans was investigated with respect to its possible use in utilization of industrial wastes containing lactose. Six stablelac + mutants were obtained after the treatment withN-methyl-N′-nitroso-N′-nitroguanidine. The mutants were compared with the parent strain.(More)
Growth processes and biosynthesis of the exocellular polyglucan-type polysaccharide, produced by the bacteria Achromobacter delicatulus, were studied in the laboratory fermentation apparatus FU-6 under three completely different aeration systems. The purpose of this study was to find the most economical way of the polysaccharide biosynthesis. The growth(More)
The zone near to plant leaves, colonized by many different groups of microorganisms such as bacteria, yeasts and moulds is called the phyllosphere (since 1970 when the 1st symposium was held in England, organized by T.F. Preece and C.H. Dickinson). The book contains papers presented at the 4th symposium on the microbiology of the phyllosphere in 1985. The(More)
Detoxication of 2,4-dichlorophenoxyacetic acid (2,4-D) in samples of chernozem soil was determined by a biological test and the time course of production of14CO2 a product of microbial degradation of 2-14C-2,4-D, was measured during 38-d incubation at 28°C in the dark. Enrichment of the soil with glucose (1000 ppm), two exocellular bacterial glucan and(More)
The cell-growth kinetics and study of exocellular polysaccharide production of the mutant strain Achromobacter delicatulus AD39 in the laboratory flasks or fermentors in submersion conditions have been demostrated. The maximum of the polymer production occured in the first period of stationary culture growth phase, with the yield 1.0–1.7% w/v.
A novel mutant strain ofAchromobacter delicatulus was cultivated in a 75-L fermentor, using whey as carbon source and production of exocellular glucan was measured. The fermentation had a biphasic character: growth was followed by the phase of polysaccharide production, the maximum of which was 17 g/L.