Josie Lambourdière

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The primary purpose of DNA-barcoding projects is to generate an efficient expertise and identification tool. This is an important challenge to the taxonomy of the 21st century, as the demand increases and the expert capacity does not. However, identifying specimens using DNA-barcodes requires a preliminary analysis to relate molecular clusters to available(More)
UNLABELLED PREMISE OF THE STUDY Microsatellite loci were developed for the understudied root crop yam bean (Pachyrhizus spp.) to investigate intraspecific diversity and interspecific relationships within the genus Pachyrhizus. • METHODS AND RESULTS Seventeen nuclear simple sequence repeat (SSR) markers with perfect di- and trinucleotide repeats were(More)
PREMISE OF THE STUDY Viola elatior (Violaceae) is a Eurasian perennial plant species in which French populations are threatened by anthropogenic pressures. Microsatellite primers were developed to investigate its genetic structure and diversity. METHODS AND RESULTS Eight microsatellite markers were isolated using next-generation sequencing. Loci were(More)
A multiplex PCR assay was developed for the identification of polymorphic microsatellite loci (8) for a North African toad, Amietophrynus mauritanicus, using 454 GS-FLX Titanium pyrosequencing. The utility of these markers was evaluated in 40 individuals from two different Moroccan populations. Number of alleles per locus ranged from 3 to 15, with expected(More)
BACKGROUND The European bison (Bison bonasus), now found in Europe and the Caucasus, has been proposed to originate either from the extinct steppe/extant American bison lineage or from the extinct Bison schoetensacki lineage. Bison schoetensacki remains are documented in Eurasian Middle Pleistocene sites, but their presence in Upper Pleistocene sites has(More)
The detection of hybridization among freshwater fish species is of main concern for conservation programs and fish farming. We assessed the incidence of natural hybridization between two vulnerable species of Andean pupfishes (Cyprinodontidae; Orestias agassizii and O. luteus) that represent an important component of local fisheries. We combined(More)
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