Joshua M Barnett

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PURPOSE The efficacy of three matrix metalloproteinase (MMP) inhibitors with various selectivities (Ro-31-9790, AG3340, and DPC-A37668) was investigated in a rat model of retinopathy of prematurity, to examine the roles of MMP-2 and -9 in retinal neovascularization. The susceptibilities of MMP-2(-/-) and -9(-/-) mice to preretinal neovascularization were(More)
PURPOSE To characterize the angiostatic effect of penetrating ocular injury and to begin to explore its mechanism, with an emphasis on the role of pigment epithelium-derived factor (PEDF). METHODS Using the rat model of oxygen-induced retinopathy (OIR), single or multiple dry needle injuries were made, penetrating the globe of one eye; the opposite eye(More)
PURPOSE PGE(2) binds to PGE(2) receptors (EP(1-4)). The purpose of the present study was to investigate the role of the EP(4) receptor in angiogenic cell behaviors of retinal Müller cells and retinal microvascular endothelial cells (RMECs) and to assess the efficacy of an EP(4) antagonist in rat models of oxygen-induced retinopathy (OIR) and laser-induced(More)
PURPOSE To identify and characterize the role of cytosolic phospholipase A(2) (cPLA(2)) in retinal angiogenesis using relevant cell-based assays and a rodent model of retinopathy of prematurity. METHODS The phosphorylation states of cPLA(2) and p38 MAP kinase and the expression of COX-2 were assessed by Western blot analysis in rat Müller cells. The(More)
PURPOSE We investigated endoglin expression in hypoxic microvascular endothelial cells and retinal endoglin expression in rats that develop experimental oxygen-induced retinopathy (OIR). We also tested neutralizing antibodies (Abs) against endoglin (anti-CD105 Ab) and VEGF (anti-VEGF Ab) either alone or in combination for efficacy against serum-induced(More)
Rats were trained in a semi-automated Y maze to find food at the end of the lighted arm. Those treated with 10 μ g lysine vasopressin, 90 min before training learned the response to a 9 10 correct choice criterion significantly faster than saline treated animals. There was no difference in rate of forgetting between the treatment groups, as evidenced by a(More)
Chicks were trained to avoid pecking either a red or a blue bead in a one-trial avoidance task by coating one bead with methy anthranilate. They avoided the aversant bead on retention tests 10 to 180 min or 24 hr after learning, but not the neutral bead. Intracranial administration of ouabain or cycloheximide (CXM) 5 min before learning resulted in decay in(More)
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