Joshua G Pierce

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A 42-kilodalton cytoplasmic membrane protein is synthesized when high CO(2)-grown cells of Synechococcus PCC 7942 (Anacystis nidulans R2) are exposed to low CO(2). The structural gene for this protein (cmpA) has been cloned and sequenced and shown to encode a 450 amino acid polypeptide with a molecular mass of 49 kilodalton. A deletion mutant lacking the(More)
Ribulose bisphosphate carboxylase is essential for both photoautotrophic and photoheterotrophic growth of the cyanobacterium Synechocystis 6803. However, a mutant lacking cyanobacterial carboxylase could be obtained by replacing the natural carboxylase gene with the corresponding gene from Rhodospirillum rubrum, a photosynthetic anaerobe. This treatment(More)
The glycopeptide antibiotics are the most important class of drugs used in the treatment of resistant bacterial infections including those caused by methicillin-resistant Staphylococcus aureus (MRSA). After more than 50 years of clinical use, the emergence of glycopeptide-resistant Gram-positive pathogens such as vancomycin-resistant enterococci (VRE) and(More)
The oligosaccharides of the bovine pituitary gonadotropin lutropin are N-linked to asparagine residues. These carbohydrates are unusual in that, although they contain the mannose, N-acetylglucosamine, and fucose typical of N-linked oligosaccharides, they also contain one residue of N-acetylgalactosamine but insignificant amounts of sialic acid or galactose.(More)
An cu-L-fucosidase has been purified from rat epididymis by fractionation of extracts with ammonium sulfate and subsequent chromatography on ion exchange celluloses. It is a glycoprotein with a molecular weight of 210,000 to 220,000 and dissociates in the presence of mercaptoethanol and guanidine hydrochloride or sodium dodecyl sulfate. The dissociated(More)
Further characterization of the free alpha subunit immunoreactive material, not combined with beta subunit in extracts of bovine pituitaries, shows that the only significant modifications, relative to alpha subunits themselves, are the oligosaccharide O-linked to threonine-43, and heterogeneity of the carboxyl terminus. Removal of the O-linked carbohydrate(More)
The objective was to assess the effectiveness of antiserums to the B-subunit of bovine TSH (thyrotropin) and aand p-subunits of LH (luteinizing hormone) for immunochemical staining of rat pituitary cells and in so doing to acquire more information about the properties of the subunits. For the following reasons it was concluded that antiserum to TSH-/3 is(More)