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Cholesterol inhibition of pentacyclic triterpenoid biosynthesis in Tetrahymena pyriformis.
SYNOPSIS. Tetrahymena pyriformis synthesizes tetrahymanol and “diplopterol” from acetate, mevalonate or squalene. The formation of these pentacyclic triterpenoid alcohols is inhibited by the additionExpand
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Rotational isomerism about the 17(20)-bond of steroids and euphoids as shown by the crystal structures of euphol and tirucallol.
The influence of configuration at C-20 on rotation about the 17(20)-bond in steroids and euphoids was examined by x-ray crystallographic studies of the C-20 epimers euphol and tirucallol. The H atomExpand
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Desmosterol as the Major Sterol in L-Cell Mouse Fibroblasts Grown in Sterol-Free Culture Medium
The principal sterol synthesized by L-cell mouse fibroblasts is desmosterol. Cholesterol was not detected in these cells when they were grown in a sterol-free culture medium. These findings indicateExpand
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Ergosterol replacement of tetrahymanol in Tetrahymena membranes.
Abstract The naturally occurring pentacyclic triterpene alcohol of Tetrahymena pyriformis , tetrahymanol, is replaced by ergosterol as a result of supplementation of the growth medium with thatExpand
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Effect of sterol replacement in vivo on the fatty acid composition of Tetrahymena.
The addition of ergosterol to cultures of Tetrahymena pyriformis results in (a) the accumulation of the sterol by the cells; (b) the inhibition of the synthesis of the pentacyclic triterpenoidExpand
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The metabolism of stigmasterol and cholesterol by Paramecium aurelia.
Abstract 1. 1. Paramecium aurelia , stock 299, can be grown in axenic culture only when supplemented with fatty acids and an appropriate sterol. The metabolic fate of nutritionally activeExpand
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Conformational analysis of enzyme-substrate complex in the dehydrogenation of sterols by Tetrahymena pyriformis.
Abstract Both ergosta-5,24(28)-dien-3β-ol and stigmasta-5,trans-24(28)-dien-3β-ol were metabolized in Tetrahymena pyriformis to give the respective Δ5,7,22,24(28)-tetraenes, vis.Expand
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The steric requirements for the metabolism of sterols by Tetrahymena pyriformis.
tives; however, the organism did not significantly metabo- lize the “left-handed” (Z)-17(20)-dehydrocholesterol in which C-22 is c&-oriented with respect to C-13. We interpret this to imply aExpand
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