Joseph M. Perez

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Vegetable oils can contribute toward the goal of energy independence and security due to their naturally renewable resource. They are promising candidates as base fluids for ecofriendly lubricants because of their excellent lubricity, biodegradability, good viscosity-temperature characteristics, and low evaporation loss. Their use, however, is restricted(More)
The cysK gene encoding a cysteine synthase of Geobacillus stearothermophilus V was overexpressed in E. coli and the recombinant protein was purified and characterized. The enzyme is a thermostable homodimer (32 kDa/monomer) belonging to the beta family of pyridoxal phosphate (PLP)-dependent enzymes. UV-visible spectra showed absorption bands at 279 and 410(More)
Biobased synthetic fluids are preferred alternatives to petroleum-based products due to their nontoxic and ecofriendly nature. Recent developments in biobased synthetic lubricants are a result of their comparable performance properties with mineral oils for industrial and automotive applications. These synthetic fluids can be chemically custom designed for(More)
The O-antigen determines the specificity of bacterial serotype, a sort of bacterial fingerprinting. In this work we report the extraction, purification and characterization of the O-antigen of two pathogenic bacteria, Escherichia coli O16 and Salmonella typhimurium. Molecular fingerprints found in the vibrational spectra represent a powerful analytical(More)
The biodegradable properties and lubricating ability of greases depend on both the base oil and the thickener. Mineral oils are the most widely used lubricant base fluids due to their inherent lubricity and low cost, but recent environmental awareness has forced consideration of the use of biodegradable fluids such as vegetable oils and certain synthetic(More)
We introduce a new image cytometer design for the detection of very small particulates and demonstrate its capability in water analysis. The device is a compact microscope composed of off-the-shelf components, such as a light emitting diode (LED) source, a complementary metal-oxide-semiconductor (CMOS) image sensor, and a specific combination of optical(More)
The salt-induced B- to Z-DNA conformational transition is a cooperative- and time-dependent process. From a modified form of the logistic equation which describes an equilibrium between two states we have deduced a kinetic function to quantify the degree of the B to Z transition of a synthetic (dG-dC) ⋅ (dG-dC) polynucleotide. This function was obtained by(More)
Vegetable oils have significant potential as a base fluid and a substitute for mineral oil for grease formulation. This paper describes the preparation of biobased grease with high oxidative stability and a composition useful for industrial, agriculture/farming equipment, and forestry applications. The process utilizes more oxidatively stable epoxy(More)
The gamma-ray imaging using coded aperture masks as focusing elements is an extended technique for static position sensitive detectors. Several transfer functions have been proposed to implement mathematically the set of holes in the mask, the uniformly redundant array collimator being the most popular design. A considerable amount of work has been done to(More)