Joseph M. Kornfeld

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Cell walls were prepared mechanically from both hyphae and conidia of Penicillium chrysogenum. The major carbohydrate components of cell wall hydrolysates were glucose, galactose and glucosamine, with lesser amounts of mannose and rhamnose. The conidia were richer in galactose and the hyphae contained more glucosamine. The distribution of the monomers in(More)
A quantitative microagglutination test for measuring group A streptococcal antibody levels is described and evaluated. The pro­ cedure requires only a patient’s serum and a standardized antigen consisting of pepsin and trypsin treated group A streptococcal cells. The antibody measured appears to be directed mainly at the group A carbohydrate of the cell(More)
The fine structure of resting and germinating conidia ofPenicillium chrysogenum has been examined by electron microscopy. In addition to enlargement of the cells, a number of changes in ultrastructure become evident as morphogenesis proceeds. The newly synthesized germ tube is continuous with the corresponding layers of the conidial wall. Some conidial wall(More)
The preparation of ribosomal particles from P. chrysogenum has been described. After correction to 20°C and extrapolation to infinite dilution, they had sedimentation coefficients of approximately 80S, 60 S, and 40 S. The washed ribosomes plus purified supernatant fraction, KCl and sucrose incorporated C14-l-amino acids into protein. There was no(More)