Joseph E De Larco

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TPA (12-O-tetradecanoyl-phorbol-13-acetate) reversibly inhibits the binding of (125)I-labelled epidermal growth factor (EGF) to treated mouse and human cells, but does not affect the binding of various other ligands to their membrane receptors. It alters the affinity of the receptors for EGF without changing the total number of available receptors per cell.(More)
Purified mouse nerve growth factor (NGF)radiolabeled with 125I was tested for its ability to bind to a variety of different cultured cells. NGF binds readily to human and hamster melanoma cells but does not bind to many other cell lines. The three cell lines with the highest number of NGF receptors were derived from metastatic melanomas. One of these lines,(More)
In the last decade, several groups have shown a direct correlation between the inappropriate or ectopic release of interleukin (IL)-8 by tumor cells in vitro and their growth and metastatic potential using in vivo models of tumor growth. IL-8 is a potent neutrophil chemoattractant. Neutrophils, as "early responders" to wounds and infections, release enzymes(More)
Normal cells in culture have membrane receptors for epidermal growth factor (EGF); EGF stimulates cells to divide by binding to these receptors. Cells transformed by murine and feline sarcoma viruses rapidly lose the ability to bind EGF, whereas cells transformed by the DNA tumour viruses, polyoma and SV40, or infected with non-transforming RNA tumour(More)
Data presented in this report indicate short-term in vitro treatment of nonmetastatic MCF-7 breast carcinoma cells with the chemotherapeutic agents-, Adriamycin and/or 5-fluoro-2'-deoxyuridine (FUdR), induced changes in the expressed phenotype. Cells treated sequentially with Adriamycin and FUdR expressed a metastatic phenotype. The results also show(More)
663 carcinogen, the nitrofuran FANFT (Cohen et al., 1978). It is not known how saccharin acts as a promoter, but undoubtedly tumours promoted by saccharin show a wide variety of differentiation patterns both within the tumour and in adjacent, non-neoplastic areas of the urothelium. This suggests that saccharin in some way disturbs the normal control of(More)
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