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Imprecision is well suited for representing uncertainty in choice during an engineering design process, and in particular for computer aided engineering design and analysis tools. To propagate imprecise understanding through engineering tools, however, rst the membership functions must be constructed based on the understandings of the design engineer. To(More)
Human pluripotent stem cells (hPSCs) constitute a promising resource for use in cell-based therapies and a valuable in vitro model for studying early human development and disease. Despite significant advancements in the derivation of specific fates from hPSCs, the generation of skeletal muscle remains challenging and is mostly dependent on transgene(More)
All Rights Reserved iii Acknowledgements First, I would like to thank my advisor, Rod Goodman, for providing me with the resources and tools necessary to produce this work. Next, I would like to thank the members of my thesis defense committee, Martin, and Chris Diorio. I would also like to thank Petr Vogel for being on my candidacy committee. Thanks also(More)
In skeletal muscle cells, GLUT1 is responsible for a large portion of basal uptake of glucose and dehydroascorbic acid, both of which play roles in antioxidant defense. We hypothesized that conditions that would decrease GLUT1-mediated transport would cause increased reactive oxygen species (ROS) levels in L6 myoblasts, while conditions that would increase(More)
OBJECTIVE The glucose and dehydroascorbic acid (DHA) transporter GLUT1 contains a phosphorylation site, S490, for ataxia telangiectasia mutated (ATM). The objective of this study was to determine whether ATM and GLUT1-S490 regulate GLUT1. RESEARCH DESIGN AND METHODS L6 myoblasts and mouse skeletal muscles were used to study the effects of ATM inhibition,(More)
Preliminary design is characterized by imprecision: the designer's uncertainty in choosing among alternatives. For any computation to occur, these informal descriptions must be translated into formal model representations, usually in the form of constraints. Conventional constraint based CAD systems are used to manipulate input and output variables, by(More)