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Excitatory postsynaptic currents (EPSCs) were recorded extracellularly from large muscle fibers by means of 'patch clamp' electrodes. Compared to usual extracellular recordings, better signal/noise ratio and temporal stability were achieved. In the range of extracellular calcium concentrations [Ca]0 between 2.7 and 13.5 mmol/l (normal), the average(More)
We studied the kinetics of the unedited version of rat GluR6 glutamate (glu) receptor channels, GluR6Q, in outside-out patches using a system for submillisecond solution exchange. Half-maximum activation of the channels was reached with approximately 0.5 microM glu. The maximum slope of the double-logarithmic plot of the peak current versus glu was(More)
In outside-out patches of mouse-muscle membrane, embryonic-like channels were activated by pulses of acetylcholine (ACh). On increasing the ACh concentration, the rate of desensitization, 1/tau d, increased linearly with the peak open probability, indicating desensitization from the open state. Desensitization had only one time constant tau d at each ACh(More)
Release and facilitated release of transmitter at neuromuscular junctions of the crayfish Astacus were measured as a function of [Ca]0 at single junctions using a patch clamp technique. Tests were made of a quantitative model that relates release of transmitter to [Ca]i. The model assumes three processes, entry of Ca during the action potential, release of(More)
In the present study, the activation of rat recombinant alpha(1)beta(2)gamma(2S) gamma-aminobutyric acid (GABA)-ergic Cl(-) channel expressed in human embryonic kidney (HEK) 293 cells by ivermectin was investigated. Maximal activation of the channel occurred with GABA concentrations of 10 mM or 20 microM ivermectin both achieving about the same current(More)
Quantal transmitter release was measured in small portions of neuromuscular junctions by means of a perfused macro-patch-clamp electrode. Release was elicited by graded current pulses through the recording electrode (excitation blocked by TTX). On increasing the stimulation current from a threshold amplitude, release rose steeply for several orders of(More)
Transmitter quanta were elicited from nerve terminals of crayfish and frog muscle by depolarization pulses through a macro-patch-clamp electrode. The rates of quantal release for twin pulses and their ratio, twin pulse facilitation, Fd, were determined. When the electrode was perfused with normal Cae (13.5 mM for crayfish, 1.8 mM for frog), Fd was low for(More)
In lobster and crayfish neuromuscular junctions superfusion of the excitatory transmitter L-glutamate (L-Glu) has been shown to depress release from motor axon terminals. In crayfish the effect depended on the level of depolarization of the terminal, Glu facilitating release when large depolarizing stimuli were applied. The presynaptic inhibition by Glu(More)
Quantal synaptic currents were recorded at nerve terminations on the opener muscle of crayfish using a macro-patch-clamp electrode, and the release was elicited by depolarizing current pulses applied to the terminal through the same electrode. After 2 ms depolarization pulses at low temperature, release started with about 2 ms delay after the onset of(More)
In excitatory neuromuscular junctions of crayfish quantal synaptic currents were recorded focally by means of a macro-patch-clamp electrode. Through the same electrode the nerve terminal was depolarized by current pulses which elicited quantal postsynaptic currents (pEPSCs). The terminals were electrically inexcitable and the quantum content (m) of pEPSCs(More)