Josane F. Sousa

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BACKGROUND Melanoma progression occurs through three major stages: radial growth phase (RGP), confined to the epidermis; vertical growth phase (VGP), when the tumor has invaded into the dermis; and metastasis. In this work, we used suppression subtractive hybridization (SSH) to investigate the molecular signature of melanoma progression, by comparing a(More)
BACKGROUND The ongoing efforts to sequence the honey bee genome require additional initiatives to define its transcriptome. Towards this end, we employed the Open Reading frame ESTs (ORESTES) strategy to generate profiles for the life cycle of Apis mellifera workers. RESULTS Of the 5,021 ORESTES, 35.2% matched with previously deposited Apis ESTs. The(More)
BACKGROUND The sequencing of the D.melanogaster genome revealed an unexpected small number of genes (~ 14,000) indicating that mechanisms acting on generation of transcript diversity must have played a major role in the evolution of complex metazoans. Among the most extensively used mechanisms that accounts for this diversity is alternative splicing. It is(More)
Myosin-V is involved in organelle and vesicle trafficking in Saccharomyces cerevisiae and in other eukaryotic cells from yeast to human. In the present study, we determined by FACS that the major subpopulations of the peripheral blood mononuclear cells express myosin-V with similar fluorescence intensity. Confocal microscopy showed intense labeling for(More)
Melanoma is a highly aggressive and therapy resistant tumor for which the identification of specific markers and therapeutic targets is highly desirable. We describe here the development and use of a bioinformatic pipeline tool, made publicly available under the name of EST2TSE, for the in silico detection of candidate genes with tissue-specific expression.(More)
Previous studies proposed that myosin-Va regulates apoptosis by sequestering pro-apoptotic Bmf to the actin cytoskeleton through dynein light chain-2 (DLC2). Adhesion loss or other cytoskeletal perturbations would unleash Bmf, allowing it to bind and inhibit pro-survival Bcl2 proteins. Here, we demonstrated that overexpression of a myosin-Va medial tail(More)
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. ABSTRACT Objectives Spasmolytic polypeptide-expressing metaplasia (SPEM)(More)
TO THE EDITOR Melanoma is a highly metastatic and therapeutically resistant cancer, whose incidence has more than tripled in the last decades (Smalley et al., 2010). Physiologically, melanocytes produce and store melanin pigments in the melanosomes, which are transported to the cell periphery and transferred to keratinocytes, a process that requires the(More)
Reference Tissue Type Dyrskjøt et al. (2004) 1 Urinary blader carcinoma in situ lesion and muscle invasive carcinoma Talantov et al. (2005) 2 Melanoma and benign nevus Kuriakose et al. (2005) 3 Head and neck squamous cell carcinoma Liu et al. (2006) 4 Prostate carcinoma Cheng et al. (2008) 5 Breast carcinoma and Invasive ductal carcinoma 1-Dyrskjøt L,(More)
Background: Melanoma progression occurs through three major stages: radial growth phase (RGP), confined to the epidermis; vertical growth phase (VGP), when the tumor has invaded into the dermis; and metastasis. In this work, we used suppression subtractive hybridization (SSH) to investigate the molecular signature of melanoma progression, by comparing a(More)
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