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Normalisation to a reference gene is the most common method of internally controlling for error in quantitative PCR (qPCR) experiments. Studies based on qPCR in chickpea have been carried out using potential reference genes exclusively. Inappropriate normalisation may result in the acquisition of biologically irrelevant data. We have tested the expression(More)
The advent of affordable Next Generation Sequencing technologies has had major impact on studies of many crop species, where access to genomic technologies and genome-scale data sets has been extremely limited until now. The recent development of genomic resources in blueberry will enable the application of high throughput gene expression approaches that(More)
Blueberry is a high-value crop with recognized nutritional characteristics that has led to an increase in consumer demand over the last several years. With its increasing agricultural and commercial importance, genetic and genomic tools have recently become available for use in characterizing its genetic diversity and in molecular breeding strategies. Here,(More)
An essential step in the carotenoid biosynthesis pathway is the formation of phytoene by phytoene synthase (PSY). In this study, three new genes coding PSYs (CpPSYA, CpPSYB, and CpPSYC) were cloned from Cucurbita pepo and their expression patterns analysed in three cultivars of summer squash which had a different carotenoid content. The gene sequences had a(More)
The rumen is lined on the luminal side by a stratified squamous epithelium that is responsible for not only absorption, but also transport, extensive short-chain fatty acid (SCFA) metabolism and protection. Butyrate has been demonstrated to initiate the differentiation of the tissue following introduction of solid feed to the weaning neonate as well as(More)
The qPCR assay has become a routine technology in plant biotechnology and agricultural research. It is unlikely to be technically improved, but there are still challenges which center around minimizing the variability in results and transparency when reporting technical data in support of the conclusions of a study. There are a number of aspects of the pre-(More)
Parasitic angiosperms are a pest for many crops in the World. Broomrapes (Orobanche spp.) belong to this group of parasites and attach to the roots of the hosts in order to take water and nutrients from them. This causes a pernicious effect on the crops which can lead to a complete yield loss in some cases. No complete control measures are available to(More)
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