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Exophilin8/MyRIP/Slac2-c is an effector protein of the small GTPase Rab27a and is specifically localized on retinal melanosomes and secretory granules. We investigated the role of exophilin8 in insulin granule trafficking. Exogenous expression of exophilin8 in pancreatic β cells or their cell line, MIN6, polarized (exophilin8-positive) insulin granules at(More)
The carbohydrate antigens Tn and sialyl-Tn (STn) are expressed in most carcinomas and usually absent in healthy tissues. These antigens have been correlated with cancer progression and poor prognosis, and associated with immunosuppressive microenvironment. Presently they are used in clinical trials as therapeutic vaccination, but with limited success due to(More)
Striking evidence associates cancer stem cells (CSCs) to the high recurrence rates and poor survival of patients with muscle-invasive bladder cancer (BC). However, the prognostic implication of those cells in risk stratification is not firmly established, mainly due to the functional and phenotypic heterogeneity of CSCs populations, as well as, to the(More)
Group leader of the 'Cilia Regulation and Disease' laboratory currently hosting 2 Postdocs, 2 PhD students, 1 technician and 2 Master students. FUNDING 2012-2016 FCT-ANR/BEX-BID/0153/2012-Quantitative analysis of asymmetric gene expression in the left-right organizer in response to flow forces: what comes downstream of cilia driven flow? Ongoing. 249,000€(More)
Cancer arises in the twenty-first century as one of the leading causes for mortality in the western civilization. In the last decades, several genes were identified as important players in the transformation of a normal cell into a tumor cell. Therefore, modulation of those genes is a promising strategy for cancer treatment. Gene downregulation can be(More)
Purtx~se: We like to demonstrate glycation of S,-, R,-and y-crystallins in the microsectioned bovine lens, by isoelectric focusing using three staining methods. Methods: Pour bovine lenses of 1.185+0.131 years were frozen-sectioned into equa'cor and 11 layers. Water-soluble crvstallins were seuarated bv isoelectric focusing an8 stained: 1. with ?oomassie(More)
Purpose. Bendazao has been used as an anticataraotogenic drug. It has been repotted that this sots by preventing protein denaturation. In this study the ability d 'bendazac to inhibit in vitro glyoatlln ot hum;,n lens crystallinswas evakrated. Methods. Possible effects of bendazac: were detected by incubation of WS crystallins with the reducing sugars(More)
To measure primaty and secondary products of lipid Purnose oxidation with age in human lens membranes. Lipid oxidation is believed to contribute to a number of senescent diseases including cataract. Secondary products of lipid oxidation, such as malondialdehyde, have been measured in the lens and are not a good indicator of lipid oxidation. hence primaly(More)
Purpose: Lipid peroxidation has recently been associated to a number of agc-related diseases including cataract development. This study was designed to establish the best conditions to adapt a technical approach based on the incorporation of a fluorescent pmbe (patina& acid) into biological membranes to monitor early stages of lipid pemxidation. The method(More)
Purpose: Lipid peroxidation has recently been associated to a number of agc-related diseases including cataract development. This study was designed to establish the best conditions to adapt a technical approach based on the incorporation of a fluorescent pmbe (patina& acid) into biological membranes to monitor early stages of lipid pemxidation. The method(More)