José María Chaves

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The purpose of the study was to compare the effects of deamidation alone, truncation alone, or both truncation and deamidation on structural and functional properties of human lens alphaA-crystallin. Specifically, the study investigated whether deamidation of one or two sites in alphaA-crystallin (i.e., alphaA-N101D, alphaA-N123D, alphaA-N101/123D) and/or(More)
The purpose of the study was to determine the effects of truncation of various regions of betaB1-crystallin on its structural properties and stability of heterooligomers formed by wild-type (WT) betaB1 or its deletion mutants with WT betaA3-crystallin. For these analyses, seven deletion mutants of betaB1-crystallin were generated with the following(More)
PURPOSE The purpose was to characterize the properties of a proteinase activity associated with betaA3-crystallin, which was isolated from the alpha-crystallin fraction of human lenses. METHODS An inactive, Arg-bond hydrolyzing proteinase in the alpha-crystallin fraction, which was isolated from the water soluble (WS) protein fraction of 60- to(More)
The purpose of the study was to identify non-covalently held complexes that exist in the water-soluble high molecular weight (WS-HMW) protein fractions of normal human lenses of 20-year-old and 60- to 70-year-old, and in the age-matched 60- to 70-year-old cataractous lenses. The WS protein fractions were prepared from five pooled normal lenses of(More)
Pieces of lumbar adipose tissue from 19-day pregnant rats and their virgin controls were incubated in the presence of albumin, glucose, U-14C-glycerol, and either bovine insulin (200 microU/ml) or adrenaline (2.6 microM). The rate of glycerol release in the medium was augmented in both fed and 48-hour fasted pregnant rats. Insulin reduced this parameter in(More)
Prosthetic joint infection (PJI) due to Brucella spp. is extremely rare. We report the case of a prosthetic hip infection due to Brucella melitensis in a 51-year-old male patient. The initial presentation was a gluteal abscess. There was radiographic evidence of implant loosening. The patient was cured after prolonged treatment with streptomycin,(More)
Pregnant rats at 12 and 21 days of gestation and their virgin controls were injected intravenously with U-14C-glycerol and decapitated 1, 3, or 10 min later. The conversion of labelled glycerol to 14C-glucose was augmented in the 21-day pregnant rats. The disappearance of the newly formed 14C-glucuse from blood was faster in both 12- and 21-day pregnant(More)
The aim of this study was to determine relative importance of N-terminal domain and C-terminal extension of αA-crystallin during their in vitro complex formation with phakinin and filensin (the two lens-specific intermediate filament [IF] proteins). Cloned phakinin, filensin and vimentin were purified under a denaturing conditions by consecutive(More)
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