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The sophistication and complexity of analysis performed by today's network intrusion prevention systems (IPSs) benefits greatly from implementation using general-purpose CPUs. Yet the performance of such CPUs increasingly lags behind that necessary to process today's high-rate traffic streams. A key observation, however, is that much of the traffic(More)
Stateful, in-depth, inline traffic analysis for intrusion detection and prevention is growing increasingly more difficult as the data rates of modern networks rise. Yet it remains the case that in many environments, much of the traffic comprising a high-volume stream can, after some initial analysis, be qualified as of "likely uninteresting." We present a(More)
The structure of many standalone network intrusion detection systems (NIDSs) centers around a chain of analysis that begins with packets captured by a packet filter, where the filter describes the protocols (TCP/UDP port numbers) and sometimes hosts or subnets to include or exclude from the analysis. In this work we argue for augmenting such analysis with(More)
In the trabecular meshwork (TM) of the eye, regulation of tissue contractility by the PPRARI sequence within the Heparin II (HepII) domain of fibronectin is believed to control the movement of aqueous humor and dictate the level of intraocular pressure. This study shows that the HepII domain utilizes activated α4β1 integrin and collagen to mediate a(More)
PURPOSE To directly visualize the live cellularity of the intact human trabecular meshwork (TM) and quantitatively analyze tissue viability in situ. METHODS Human donor corneoscleral rims were sectioned immediately before intravital dye incubation to label nuclei (Hoechst 33342 & propidium iodide [PI]); cytosol (CellTracker Red CMTPX, calcein AM); and(More)
Actomyosin contractility modulates outflow resistance of the aqueous drainage tissues and intraocular pressure, a key pathogenic factor of glaucoma. We established methodology to reliably analyze the effect of latrunculin-B (Lat-B)-induced actin depolymerization on outflow physiology in live mice. A voltage-controlled microperfusion system for delivering(More)
The contractile trabecular meshwork (TM) modulates aqueous humor outflow resistance and intraocular pressure. The primary goal was to visualize and quantify human TM contractile state by analyzing actin polymerization (F-actin) by 2-photon excitation fluorescence imaging (TPEF) in situ. A secondary goal was to ascertain if structural extracellular matrix(More)
PURPOSE To characterize the three-dimensional (3-D) structure of the human trabecular meshwork (TM) by two-photon excited (TPEF) autofluorescence (AF) and optical sectioning without conventional histologic embedding and sectioning. METHODS Viable human ex vivo explants of the anterior chamber angle containing the aqueous humor drainage tissue in situ were(More)
PURPOSE To determine whether the heparin II (HepII) domain of fibronectin previously shown to increase outflow facility affects the formation and assembly of actin cytoskeleton and adherens junctions in human trabecular meshwork (HTM) cells. METHODS Normal HTM cells and two transformed HTM cell lines were treated for 24 hours with increasing(More)