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Structure of the connector of bacteriophage T7 at 8A resolution: structural homologies of a basic component of a DNA translocating machinery.
The three-dimensional structure of the bacteriophage T7 head-to-tail connector has been obtained at 8A resolution using cryo-electron microscopy and single-particle analysis from purified recombinantExpand
Visualization of Single Escherichia coli FtsZ Filament Dynamics with Atomic Force Microscopy*
Experimental evidence is provided supporting the model of single-strand polymerization plus cyclization recently proposed to explain the hydrodynamic behavior of the polymers in solution. Expand
Distinct Gene Expression Profiling after Infection of Immature Human Monocyte-Derived Dendritic Cells by the Attenuated Poxvirus Vectors MVA and NYVAC
Following MVA or NYVAC infection in MDDC, similarities as well as differences are found between these virus strains in the expression of cellular genes with immunological function, which should have an impact when these vectors are used as recombinant vaccines. Expand
A poxvirus Bcl-2-like gene family involved in regulation of host immune response: sequence similarity and evolutionary history
The sequence similarity existing among poxvirus A46, N1, N2 and C1 protein families is described, which indicates an extensive gene gain in ancestral viruses and a further stabilization of its gene content, and it is proposed that these protein families should be merged into a single one. Expand
Essential Cell Division Protein FtsZ Assembles into One Monomer-thick Ribbons under Conditions Resembling the Crowded Intracellular Environment*
It is proposed that, in the crowded bacterial cytoplasm under assembly-promoting conditions, the FtsZ filaments tend to align forming dynamic ribbon polymers that would fit into the Z-ring even in the absence of other interactions. Expand
Cooperative behavior of Escherichia coli cell-division protein FtsZ assembly involves the preferential cyclization of long single-stranded fibrils.
A mechanism of noncooperative (isodesmic) assembly coupled with preferential cyclization of long polymers is proposed to explain the previously posed question of how a single-stranded filament of theExpand
Membrane cell fusion activity of the vaccinia virus A17–A27 protein complex
P pH‐dependent fusion activity was identified to reside in A17 amino‐terminal ectodomain after overexpression in insect cells using recombinant baculoviruses and was functionally demonstrated in mammalian cells by cytoplasmic transfer of fluorescent proteins, only when A17 and A27 were coexpressed. Expand
The three-dimensional structure of an eukaryotic glutamine synthetase: functional implications of its oligomeric structure.
The structure of the prokaryotic glutamine synthetases type I and II, key enzymes in nitrogen metabolism, was determined several years ago by X-ray diffraction, and consists of a double hexameric ring, and the reconstructed tetramer suggests that it only contains two functional active sites. Expand
Epitope Insertion at the N-Terminal Molecular Switch of the Rabbit Hemorrhagic Disease Virus T=3 Capsid Protein Leads to Larger T=4 Capsids
The calicivirus capsid protein, and perhaps that of many other viruses, thus can encode polymorphism modulators that are not anticipated from the plane sequence, with important implications for understanding virus assembly and evolution. Expand
Coronavirus derived expression systems
Abstract Both helper dependent expression systems, based on two components, and single genomes constructed by targeted recombination, or by using infectious cDNA clones, have been developed. TheExpand