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Brucellosis control and eradication requires serological tests and vaccines. Effective classical vaccines (S19 in cattle and Rev 1 in small ruminants), however, induce antibodies to the O-polysaccharide of the lipopolysaccharide which may be difficult to distinguish from those resulting from infection and may thus complicate diagnosis. Rough attenuated(More)
BACKGROUND The lipopolysaccharide is a major antigen and virulence factor of Brucella, an important bacterial pathogen. In smooth brucellae, lipopolysaccharide is made of lipid A-core oligosaccharide and N-formylperosamine O-polysaccharide. B. ovis and B. canis (rough species) lack the O-polysaccharide. RESULTS The polymorphism of O-polysaccharide genes(More)
Berry size of winegrapes has often been considered to influence wine composition and quality. The measurement of the size and weight of grapevine berries may provide important information to assess differences in ripening stage, pulp/skin ratio and phenolic content. This task is usually done by hand, so that it becomes slow, tedious and inaccurate. This(More)
In this paper we analyse a pressure stabilized, nite element method for the unsteady, incompressible Navier{Stokes equations in primitive variables; for the time discretization we focus on a fully implicit, monolithic scheme. We provide some error estimates for the fully discrete solution which show that the velocity is rst order accurate in the time step(More)
BACKGROUND The role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. The aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several Iberian wild ungulates. METHODS A multi-species indirect immunosorbent assay(More)
Brucellosis is a zoonosis caused by Brucella species. Brucellosis research in natural hosts is often precluded by practical, economical and ethical reasons and mice are widely used. However, mice are not natural Brucella hosts and the course of murine brucellosis depends on bacterial strain virulence, dose and inoculation route as well as breed, genetic(More)
Automatic detection of skin defects in citrus fruits using a multivariate image analysis approach. a b s t r a c t One of the main problems in the post-harvest processing of citrus is the detection of visual defects in order to classify the fruit depending on their appearance. Species and cultivars of citrus present a high rate of unpredictability in(More)
Competitive and standard enzyme-linked immunosorbent assays (ELISAs), rose bengal (RB), complement fixation, and agar gel immunoprecipitation with native hapten (AGID-NH) were compared by using sera from Brucella-free, Brucella melitensis-infected, and B. melitensis Rev1-vaccinated sheep. The most sensitive tests were indirect ELISA and RB, and the most(More)
The designations employed and the presentation of the material in this publication do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or(More)