José Antonio Lupiáñez

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The presence of nitric-oxide synthase (NOS) in peroxisomes from leaves of pea plants (Pisum sativum L.) was studied. Plant organelles were purified by differential and sucrose density gradient centrifugation. In purified intact peroxisomes a Ca(2+)-dependent NOS activity of 5.61 nmol of L-[(3)H]citrulline mg(-1) protein min(-1) was measured while no(More)
BACKGROUND Maslinic acid, a pentacyclic triterpene found in the protective wax-like coating of the leaves and fruit of Olea europaea L., is a promising agent for the prevention of colon cancer. We have shown elsewhere that maslinic acid inhibits cell proliferation to a significant extent and activates mitochondrial apoptosis in colon cancer cells. In our(More)
Chemoprevention is a pragmatic approach to reduce the risk of colorectal cancer, one of the leading causes of cancer-related death in western countries. In this regard, maslinic acid (MA), a pentacyclic triterpene extracted from wax-like coatings of olives, is known to inhibit proliferation and induce apoptosis in colon cancer cell lines without affecting(More)
Triterpenoids are known to induce apoptosis and to be anti-tumoural. Maslinic acid, a pentacyclic triterpene, is present in high concentrations in olive pomace. This study examines the response of HT29 and Caco-2 colon-cancer cell lines to maslinic-acid treatment. At concentrations inhibiting cell growth by 50-80% (IC50HT29=61+/-1 microM, IC80HT29=76+/-1(More)
We have studied the nucleic acid concentrations and protein turnover rates, fractional protein synthesis (K S), degradation (K D), and accumulation (K G) rates, synthesis capacity (C S), synthesis efficiency (K RNA), synthesis per DNA unit (K DNA), and protein retention efficiency (PRE) in the liver of rainbow trout (Oncorhynchus mykiss) at different stages(More)
Maslinic acid is a triterpene present in a considerable proportion in solid residues from olive-oil production. In the present work the effects of maslinic acid on growth, protein-turnover rates and nucleic-acid concentration on liver were investigated in the rainbow trout. Five groups of 120 fish of a mean body mass of 20 g were fed for 225 days with diets(More)
Little is known about the way in which carnivorous fish such as salmonids mobilise and metabolise dietary carbohydrates, which are essential to lipid metabolism. Thus we have studied changes caused by the absence of dietary carbohydrates to the kinetics and molecular behaviour of the four cellular NADPH-production systems [glucose 6-phosphate dehydrogenase(More)
The effect of protein quality and of supplementation of corn-gluten protein with lysine on the growth, feed conversion and protein turnover rates in white muscle was investigated in carp (Cyprinus carpio) acclimated to either 18 or 25 °C. Fish fed the lysine-deficient diet showed a significantly lower food intake, weight gain and feed-conversion efficiency(More)
We have studied the evolution of the kinetic behavior of main NADPH-generating systems, Ž. Ž. glucose-6-phosphate dehydrogenase G6PDH , 6-phosphogluconate dehydrogenase 6PGDH , Ž. Ž. malic enzyme ME and isocitrate dehydogenase NADP-dependent NADP-IDH in liver of rainbow trout at different developmental stages. Our results show that during development, liver(More)
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies(More)