José Alfonso Aguilar

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Evidence suggests that the Pseudomonas aeruginosa PAO1 gnyRDBHAL cluster, which is involved in acyclic isoprenoid degradation (A. L. Díaz-Pérez, N. A. Zavala-Hernández, C. Cervantes, and J. Campos-García, Appl. Environ. Microbiol. 70:5102-5110, 2004), corresponds to the liuRABCDE cluster (B. Hoschle, V. Gnau, and D. Jendrossek, Microbiology 151:3649-3656,(More)
Biotin-containing 3-methylcrotonyl coenzyme A (MC-CoA) carboxylase (MCCase) and geranyl-CoA (G-CoA) carboxylase (GCCase) from Pseudomonas aeruginosa were expressed as His-tagged recombinant proteins in Escherichia coli. Both native and recombinant MCCase and GCCase showed pH and temperature optima of 8.5 and 37 degrees C. The apparent K(0.5) (affinity(More)
Web engineering software development is facing continuous changes in technology implementation. This involves analysts, developers and designers to provide extra effort in the design and maintenance of Web applications in order to adapt them to changes in requirements and implementation technologies. In this paper, a systematic review is presented in order(More)
In recent years, the general interest in routing for vehicular ad hoc networks (VANETs) has increased notably. Many proposals have been presented to improve the behavior of the routing decisions in these very changeable networks. In this paper, we propose a new routing protocol for VANETs that uses four different metrics. which are the distance to(More)
Analyzing the satellite DNA in the ant species Monomorium subopacum we found two unrelated families of satellite DNA. Because these satellite DNA families were isolated using the two enzymes HaeIII and EcoRI we called the two families HaeIII and EcoRI family, respectively. The HaeIII family proved to be organized in a 135-bp basic unit repeat, the EcoRI(More)
Web designers usually ignore how to model real user expectations and goals, mainly due to the large and heterogeneous audience of the Web. This fact leads to websites which are difficult to comprehend by visitors and complex to maintain by designers. In order to ameliorate this scenario, an approach for using the i* modeling framework in Web engineering has(More)
Web design methodologies should be able to provide a requirements analysis stage to consider the large and heterogeneous audience of Web applications. In this stage, non-functional requirements (NFRs) should be addressed in order to improve the quality of the Web application perceived by users. To this aim, different configurations of requirements could be(More)
Due to the dynamic nature of the Web as well as its heterogeneous audience, web applications are more likely to rapidly evolve leading to inconsistencies among requirements during the development process. With the purpose to deal with these inconsistencies, web developers need to know dependencies among requirements considering that the understanding of(More)