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Structure of the yeast U2/U6 snRNA complex.
The U2/U6 snRNA complex is a conserved and essential component of the active spliceosome that interacts with the pre-mRNA substrate and essential protein splicing factors to promote splicingExpand
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Structural mechanisms of DNA binding and unwinding in bacterial RecQ helicases
Significance RecQ DNA helicases catalyze critical genome maintenance reactions in nearly all organisms. This study describes the crystal structure of a bacterial RecQ helicase bound in a productiveExpand
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Global shape mimicry of tRNA within a viral internal ribosome entry site mediates translational reading frame selection
Significance Viruses use alternate mechanisms to increase the coding capacity of their viral genomes. The dicistrovirus intergenic region internal ribosome entry site (IRES) adopts an RNA structureExpand
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A Non-Dicer RNase III and Four Other Novel Factors Required for RNAi-Mediated Transposon Suppression in the Human Pathogenic Yeast Cryptococcus neoformans
The human pathogenic yeast Cryptococcus neoformans silences transposable elements using endo-siRNAs and an Argonaute, Ago1. Endo-siRNAs production requires the RNA-dependent RNA polymerase, Rdp1, andExpand
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A non-Dicer RNase III and four other novel factors required for RNAi-mediated transposon suppression in the human pathogenic yeast C. neoformans
The human pathogenic yeast Cryptococcus neoformans silences transposable elements using endo-siRNAs and an Argonaute, Ago1. Endo-siRNAs production requires the RNA-dependent RNA polymerase, Rdp1, andExpand
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Structural Analysis of Multi-Helical RNAs by NMR-SAXS/WAXS: Application to the U4/U6 di-snRNA.
NMR and SAXS (small-angle X-ray scattering)/WAXS (wide-angle X-ray scattering) are highly complementary approaches for the analysis of RNA structure in solution. Here we describe an efficientExpand
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Nucleic Acid Structure Characterization by Small Angle X‐Ray Scattering (SAXS)
Small angle X‐ray scattering (SAXS) is a powerful method for investigating macromolecular structure in solution. SAXS data provide information about the size and shape of a molecule with a resolutionExpand
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Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly.
In Schizosaccharomyces pombe, transcripts derived from the pericentromeric dg and dh repeats promote heterochromatin formation via RNAi as well as an RNAi-independent mechanism involving the RNAExpand
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Spliceosome Profiling Visualizes Operations of a Dynamic RNP at Nucleotide Resolution
Tools to understand how the spliceosome functions in vivo have lagged behind advances in the structural biology of the spliceosome. Here, methods are described to globally profile spliceosome-boundExpand
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Spliceosome assembly in the absence of stable U4/U6 RNA pairing.
The cycle of spliceosome assembly, intron excision, and spliceosome disassembly involves large-scale structural rearrangements of U6 snRNA that are functionally important. U6 enters the splicingExpand
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