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Engineering the Caenorhabditis elegans Genome Using Cas9-Triggered Homologous Recombination
Study of the nematode Caenorhabditis elegans has provided important insights in a wide range of fields in biology. The ability to precisely modify genomes is critical to fully realize the utility ofExpand
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HTP-3 links DSB formation with homolog pairing and crossing over during C. elegans meiosis.
Repair of the programmed meiotic double-strand breaks (DSBs) that initiate recombination must be coordinated with homolog pairing to generate crossovers capable of directing chromosome segregation.Expand
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The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans
Experimental manipulation of protein abundance in living cells or organisms is an essential strategy for investigation of biological regulatory mechanisms. Whereas powerful techniques for proteinExpand
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RTEL1 Maintains Genomic Stability by Suppressing Homologous Recombination
Homologous recombination (HR) is an important conserved process for DNA repair and ensures maintenance of genome integrity. Inappropriate HR causes gross chromosomal rearrangements and tumorigenesisExpand
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Preventing nonhomologous end joining suppresses DNA repair defects of Fanconi anemia.
Fanconi anemia (FA) is a complex cancer susceptibility disorder associated with DNA repair defects and infertility, yet the precise function of the FA proteins in genome maintenance remains unclear.Expand
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Rapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-Conversion and Inactivation of NHEJ Repair
As in other organisms, CRISPR/Cas9 methods provide a powerful approach for genome editing in the nematode Caenorhabditis elegans. Oligonucleotides are excellent repair templates for introducingExpand
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RTEL-1 Enforces Meiotic Crossover Interference and Homeostasis
Managing Crossovers In all sexual eukaryotes a special type of cell division called meiosis produces gametes or spores. For chromosomes to segregate properly to the daughter cells during meiosis, DNAExpand
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DOG-1 Is the Caenorhabditis elegans BRIP1/FANCJ Homologue and Functions in Interstrand Cross-Link Repair
ABSTRACT Fanconi anemia (FA) is a cancer susceptibility syndrome characterized by defective DNA interstrand cross-link (ICL) repair. Here, we show that DOG-1 is the Caenorhabditis elegans homologueExpand
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BRC‐1 acts in the inter‐sister pathway of meiotic double‐strand break repair
The breast and ovarian cancer susceptibility protein BRCA1 is evolutionarily conserved and functions in DNA double‐strand break (DSB) repair through homologous recombination, but its role in meiosisExpand
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Replication blocking lesions present a unique substrate for homologous recombination
Homologous recombination (HR) plays a critical role in the restart of blocked replication forks, but how this is achieved remains poorly understood. We show that mutants in the single Rad51 paralogExpand
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