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Component-based development (CBD) technique for software has emerged to fulfill the demand on the reuse of existing artifacts. In comparison to traditional object-oriented techniques, CBD can provide more advanced abstraction concepts such as subsystem-level reusability, gross structure abstraction, and global control flow abstraction. Unfortunately,(More)
A gene encoding cysteine proteinase from Clonorchis sinensis has been cloned and expressed in Escherichia coli. The cysteine proteinase cDNA fragment was amplified by reverse transcription-polymerase chain reaction using degenerate oligonucleotide primers derived from conserved active site of cysteine proteinases. The 5' and 3' regions of the gene were(More)
SUMMARY Roles and role models have received much attention as useful concepts for developing highly reusable and dynamically evolvable systems. Role models belong to the category of collaboration-based development techniques, but most of the existing approaches to role models do not explicitly incorporate the core principle of collaboration-based(More)
Software often needs to be modified for accommodating requirements changes through the software life cycle. To deal with several accidental requirements changes related to software maintenance, a systematic and safe approach to modifying softwares is needed. The design patterns provide a high degree of design flexibility for such accidental requirements(More)
Anisakiasis is an infectious parasitic disease contracted by eating third stage larvae of Anisakis simplex (L3) carried by marine fishes. Human anisakiasis was researched for specific IgG with L3 excretory secretory products (ESP). L3ESP were prepared by daily harvesting of culture supernatant from day 2 to day 5, using two kinds culture media of RPMI-1640(More)
Software maintenance is a very expensive activity in software life cycle. As in conventional systems, Web-based applications also needs a considerable cost of maintenance efforts and in general the more cost because of their inherent complex architecture. To estimate the maintainability cost of software, many software metrics have been proposed. This paper(More)
A 7-kDa protein was purified from extracts of adult Clonorchis sinensis by a combination of ammonium sulfate precipitation, anion exchange chromatography, cation exchange chromatography, gel-filtration chromatography, and reversed-phase FPLC. The 7-kDa protein exists in the excretory-secretory products of adult C. sinensis, but not in extracts of adult(More)