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Ideal cell-free expression systems can theoretically emulate an in vivo cellular environment in a controlled in vitro platform. This is useful for expressing proteins and genetic circuits in a controlled manner as well as for providing a prototyping environment for synthetic biology. To achieve the latter goal, cell-free expression systems that preserve(More)
Computation can be performed in living cells by DNA-encoded circuits that process sensory information and control biological functions. Their construction is time-intensive, requiring manual part assembly and balancing of regulator expression. We describe a design environment, Cello, in which a user writes Verilog code that is automatically transformed into(More)
BACKGROUND Escherichia coli cell-free expression systems use bacteriophage RNA polymerases, such as T7, to synthesize large amounts of recombinant proteins. These systems are used for many applications in biotechnology, such as proteomics. Recently, informational processes have been reconstituted in vitro with cell-free systems. These synthetic approaches,(More)
BACKGROUND A large amount of recombinant proteins can be synthesized in a few hours with Escherichia coli cell-free expression systems based on bacteriophage transcription. These cytoplasmic extracts are used in many applications that require large-scale protein production such as proteomics and high throughput techniques. In recent years, cell-free systems(More)
We investigate the criterion, on the Born-Infeld background fields, for the open string pair creation to occur in Dp-(anti-)Dp-brane systems. Although the pair creation occurs generically in both Dp-Dp and Dp-anti-Dp systems for the cases which meet the criterion , it is more drastic in Dp-anti-Dp-brane systems by some exponential factor depending on the(More)
We study the superstrings suspended between a D2-and a D2-brane. We quantize the string in the presence of some general configuration of gauge fields over the (anti-)D-brane world volumes. The interstring can move only in a specific direction that is normal to the difference of the electric fields of each (anti-)D-branes. Especially when the electric fields(More)
Ideal cell-free expression systems can theoretically emulate an in vivo cellular environment in a controlled in vitro platform. 1 This is useful for expressing proteins and genetic circuits in a controlled manner as well as for providing a prototyping environment for synthetic biology. 2,3 To achieve the latter goal, cell-free expression systems that(More)
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