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EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency.
Trimethylation on H3K27 (H3K27me3) mediated by Polycomb repressive complex 2 (PRC2) has been linked to embryonic stem cell (ESC) identity and pluripotency. EZH2, the catalytic subunit of PRC2, has… Expand
An Extended Transcriptional Network for Pluripotency of Embryonic Stem Cells
Much attention has focused on a small set of transcription factors that maintain human or mouse embryonic stem (ES) cells in a pluripotent state. To gain a more complete understanding of the… Expand
FAIRE (Formaldehyde-Assisted Isolation of Regulatory Elements) isolates active regulatory elements from human chromatin.
- Paul G. Giresi, Jonghwan Kim, Ryan McDaniell, V. R. Iyer, J. Lieb
- Medicine, Biology
- Genome research
- 1 June 2007
DNA segments that actively regulate transcription in vivo are typically characterized by eviction of nucleosomes from chromatin and are experimentally identified by their hypersensitivity to… Expand
A Myc Network Accounts for Similarities between Embryonic Stem and Cancer Cell Transcription Programs
c-Myc (Myc) is an important transcriptional regulator in embryonic stem (ES) cells, somatic cell reprogramming, and cancer. Here, we identify a Myc-centered regulatory network in ES cells by… Expand
Epigenetic regulation of hematopoietic differentiation by Gfi-1 and Gfi-1b is mediated by the cofactors CoREST and LSD1.
Gfi-1 and Gfi-1b are homologous transcriptional repressors involved in diverse developmental contexts, including hematopoiesis and oncogenesis. Transcriptional repression by Gfi proteins requires the… Expand
Hypoxia-inducible factor-2α is a catabolic regulator of osteoarthritic cartilage destruction
Osteoarthritic cartilage destruction is caused by an imbalance between anabolic and catabolic factors. Here, we show that hypoxia-inducible factor-2α (HIF-2α, encoded by EPAS1) is a catabolic… Expand
A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.
- G. Hu, Jonghwan Kim, Q. Xu, Yumei Leng, S. Orkin, S. Elledge
- Biology, Medicine
- Genes & development
- 1 April 2009
We performed a genome-wide siRNA screen in mouse embryonic stem (ES) cells to identify genes essential for self-renewal, and found 148 genes whose down-regulation caused differentiation. Many of the… Expand
Global Identification of Myc Target Genes Reveals Its Direct Role in Mitochondrial Biogenesis and Its E-Box Usage In Vivo
The Myc oncoprotein is a transcription factor involved in a variety of human cancers. Overexpression of Myc is associated with malignant transformation. In normal cells, Myc is induced by mitotic… Expand
Use of in vivo biotinylation to study protein–protein and protein–DNA interactions in mouse embryonic stem cells
In gene regulation, proteins function as members of protein complexes to recognize chromosomal target DNA loci. In dissecting the pluripotent state in mouse embryonic stem (mES) cells, we have used… Expand
ETV1 directs androgen metabolism and confers aggressive prostate cancer in targeted mice and patients.
Distinguishing aggressive from indolent disease and developing effective therapy for advanced disease are the major challenges in prostate cancer research. Chromosomal rearrangements involving ETS… Expand