Jong-Kun Kim

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Laccases (EC 1.10.3.2) are copper-containing polyphenol oxidases found in white-rot fungi. Here, we report the cloning and analysis of the nucleotide sequence of a new laccase gene, fvlac7, based on the genomic sequence of Flammulina velutipes. A primer set was designed from the putative mRNA that was aligned to the genomic DNA of F. velutipes. A cDNA(More)
An electrophoretic karyotype of Korean Flammulina velutipes was obtained using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. The monokaryotic progenies (4019-20 and 4019-18) and a dikaryotic strain (4019-2018) had 6-8 chromosomes, with sizes ranging from 1.6 to 5.8 megabase (Mb) pairs. Among the 3 strains that were examined, strain(More)
Use of unnatural amino-acids in Nickel Superoxide Dismutase metallopeptide mimics to probe the influence of a key hydrogen-binding network in the metalloenzyme, Functional analysis of the metal binding sites of Staphylococcus aureus plasmid pI258 CadC Cd(II)/Zn(II)/Pb(II)-responsive repressor. metal binding sites of Na+,K+-ATPase and Ca2+-ATPase. Two Mg2+(More)
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