Jonathan S. Smythe

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Flow cytometric analysis of eosin-5-maleimide (EMA) binding to red cells is a screening test for the diagnosis of hereditary spherocytosis (HS). The present study used chemical modifications to determine the integral membrane proteins that react with EMA. The predominant interaction of EMA, contributing c. 80% of fluorescence, was with the epsilon-NH2 group(More)
Lutheran glycoprotein (Lu gp) has five predicted immuno4, and Lu13 and Au to domain 5. The organization of the LU gene was determined. The region encoding the open globulin superfamily (IgSF) domains. K562 cells were transfected with Lu cDNA and tested by flow cytometry with reading frame is arranged in 15 exons extending over ̧11 kb on chromosome 19q13.2.(More)
K562 cells were stably transfected with cDNAs encoding the band 3 found in Southeast Asian ovalocytosis (B3SAO, deletion of residues 400-408), band 3 with a transport-inactivating E681Q point mutation (B3EQ), or normal band 3 (B3). Flow cytometric analysis and quantitative immunoblotting revealed that B3SAO expressed alone was translocated to the plasma(More)
Two isoforms of the band 3 anion exchanger are expressed in mammalian cells, a 911 residue protein (B3) in red cells, and a truncated protein (KB3) in the alpha-intercalated cells of the kidney. Mutants of both isoforms are known to be associated with human disease, and mistargeting of the mutated proteins has been suggested as the mechanism of pathogenesis(More)
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