Jonathan Mitchener

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Morphologic alterations in HeLa cells deprived of serum and amino acids were studied by light and electron microscopy. After complete deprivation for 1, 3, or 6 hours, many cells contained autophagic vacuoles enclosing clearly recognizable cell organelles. The proportion of cells showing autophagy rose from 4 to 37% in the first 3 hours of deprivation. Acid(More)
A comparison between a series of splenectomies performed at the University of Virginia Medical Center for hematologic disorders between 1946 and 1962 (Series I) and 1963 and 1982 (Series II) is presented. Four hundred splenectomies (20 per year) were performed between 1963 and 1982 compared with 94 (5.5 per year) between 1946 and 1962. Also noted in Series(More)
Pancreatic DNase requires both Ca2+ and Mg2+ for its activity as measured by formation of an activated DNA template for in vitro DNA polymerase alpha assay and by the hyperchromic shift. Mn2+ can partially satisfy the Mg2+ requirement of the DNase for activation of DNA but the resulting template is only 50% as active in the DNA polymerase assay. When(More)
Intimal thickening is a significant cause of late failure of aorto-coronary vein grafts. The microscopic appearance of this thickening has some similarities to the microscopic appearance of arterial atherosclerosis, and it has been suggested that hyperlipidemia may play a role in its pathogenesis. This study examines the morphology and lipid composition of(More)
Within CAWICOMS a configuration workbench for customisable products and services will be developed which will have the following advantages versus current commercially available configuration technology: It will automate over the Web the skills of salespersons by developing adaptation and personalisation technology for inclusion in Web based configurators.(More)