Jonathan Mitchener

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Pancreatic DNase requires both Ca2+ and Mg2+ for its activity as measured by formation of an activated DNA template for in vitro DNA polymerase alpha assay and by the hyperchromic shift. Mn2+ can partially satisfy the Mg2+ requirement of the DNase for activation of DNA but the resulting template is only 50% as active in the DNA polymerase assay. When(More)
Morphologic alterations in HeLa cells deprived of serum and amino acids were studied by light and electron microscopy. After complete deprivation for 1, 3, or 6 hours, many cells contained autophagic vacuoles enclosing clearly recognizable cell organelles. The proportion of cells showing autophagy rose from 4 to 37% in the first 3 hours of deprivation. Acid(More)
A comparison between a series of splenectomies performed at the University of Virginia Medical Center for hematologic disorders between 1946 and 1962 (Series I) and 1963 and 1982 (Series II) is presented. Four hundred splenectomies (20 per year) were performed between 1963 and 1982 compared with 94 (5.5 per year) between 1946 and 1962. Also noted in Series(More)
Intimal thickening is a significant cause of late failure of aorto-coronary vein grafts. The microscopic appearance of this thickening has some similarities to the microscopic appearance of arterial atherosclerosis, and it has been suggested that hyperlipidemia may play a role in its pathogenesis. This study examines the morphology and lipid composition of(More)
We have studied nine saphenous vein bypass grafts removed from patients at the time of a second operation. Two grafts had recanalized thrombus, four had severe intimal hyperplasia and three had atherosclerotic-like lesions. In cases of intimal hyperplasia biochemical analysis of graft wall lipid content revealed a pattern identical to normal saphenous vein.(More)