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HeLa is the most widely used model cell line for studying human cellular and molecular biology. To date, no genomic reference for this cell line has been released, and experiments have relied on the human reference genome. Effective design and interpretation of molecular genetic studies performed using HeLa cells require accurate genomic information. Here(More)
Sneak circuit analysis is an important design analysis method for detecting unexpected interactions between different parts of an electrical circuit design. It demands a great deal of effort from an engineer, and is also error-prone. A method for the automation of sneak circuit analysis is described. It uses qualitative reasoning and knowledge about which(More)
We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and robust protein production platform, 'MultiBacTAG', (i) for the fluorescent labeling of target proteins and(More)
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