Learn More
The Cdt is a family of gram-negative bacterial toxins that typically arrest eukaryotic cells in the G0/G1 or G2/M phase of the cell cycle. The toxin is a heterotrimer composed of the cdtA, cdtB and cdtC gene products. Although it has been shown that the CdtA protein subunit binds to cells in culture and in an enzyme-linked immunosorbent assay (CELISA) the(More)
Chimeras composed of the cdtB gene of a novel bacterial genotoxin and the human type I DNAse I gene were constructed and their products characterized relative to the biochemical and enzymatic properties of the native proteins. The product of a cdtB/DNAse I chimera formed a heterotrimer with the CdtA and CdtC subunits of the genotoxin, and targeted mutations(More)
The periodontal pathogen Actinobacillus actinomycetemcomitans expresses a cytolethal distending toxin (CDT) that typically arrests the growth of eukaryotic cells at either the G0/G1 or G2/M phase of the cell cycle. It was previously found that CDT failed to arrest the growth of human periodontal ligament fibroblasts (HPLFs) when grown in pure culture. In(More)
Neonatal exposure to Gross murine leukemia virus results in a profound inhibition of the virus-specific T and B cell responses of adult animals. Animals exposed to virus as neonates exhibit a marked depression in virus-specific T cell function as measured by the virtual absence of in vivo delayed type hypersensitivity responses and in vitro proliferative(More)
INTRODUCTION Cytolethal distending toxin (Cdt) is potentially one of several virulence factors of Aggregatibacter actinomycetemcomitans, the prime etiological agent of localized aggressive periodontitis (LAP). Little is known regarding the Cdt-specific antibody response in humans. The current study is a quantitative and qualitative evaluation of the(More)
Mesenchymal stem cells (MSCs) are emerging donor grafts for bone regeneration in dentistry. MSCs are phenotypically and functionally skeletal site-specific based on extensive studies using human and rodent MSCs but there is paucity of information on canine MSCs (cMSCs) and their regenerative applications in veterinary dentistry. We hypothesized that cMSCs(More)
Periodontitis is a biofilm-induced chronic inflammatory disease that causes gingival inflammation and attachment loss. Destruction of periodontal tissue is a consequence of host immune inflammatory responses induced by periodontal microorganisms. Wnt5a is strongly associated with inflammatory responses and shown by several studies to be involved in(More)
AIM: To detect the presence of milk fat globule-epidermal growth factor-factor 8 (MFG-E8) in human gingival crevicular fluid (GCF) and to determine its potential role as a biomarker for periodontal disease activity. GCF was collected from a total of 230 sites from with seven subjects with gingivitis, twelve subjects with chronic moderate periodontitis,(More)
Exposure of newborn mice to Gross murine leukemia virus (GMuLV) results in persistent viral infection of the central nervous system (CNS) white matter. Animals exposed to virus as neonates showed a marked depression in GMuLV-specific B lymphocyte function as evidenced by significant decreases in adult and neonatal anti-GMuLV antibody levels.(More)
  • 1