Jonathan J. Campbell

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Stem cells are undifferentiated cells that are capable of proliferation, self-maintenance and differentiation towards specific cell phenotypes. These processes are controlled by a variety of cues including physicochemical factors associated with the specific mechanical environment in which the cells reside. The control of stem cell biology through(More)
Studies on the stem cell niche and the efficacy of cancer therapeutics require complex multicellular structures and interactions between different cell types and extracellular matrix (ECM) in three dimensional (3D) space. We have engineered a 3D in vitro model of mammary gland that encompasses a defined, porous collagen/hyaluronic acid (HA) scaffold forming(More)
The present study describes an improved fluorescent recovery after photobleaching (FRAP) technique, which has been successfully used to quantify actin dynamics within individual fibers. Chondrocytes were transfected with an eGFP-actin plasmid and cultured on glass coverslips. In cells expressing eGFP-actin, confocal microscopy was used to bleach 3 x 1(More)
Cytoskeleton is formed by three main types of filaments: microfilaments, microtubules and intermediate filaments. It is a dynamic structure which allows the cell to move, to keep its shape and to divide during mitosis. It also facilitates the intracellular transport of vesicles and organelles. The study of protein dynamics in vivo is now possible since the(More)
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