Jonathan Alexander Heiss

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Development of type 2 diabetes depends on environmental and genetic factors. We investigated the epigenome-wide association of prevalent diabetes with DNA methylation (DNAm) in peripheral blood. DNAm was measured in whole blood with the Illumina Infinium HumanMethylation450 BeadChip in two subsamples of participants from the ESTHER cohort study. Cohort 1(More)
The Illumina Infinium HumanMethylation450 BeadChip is frequently used in epigenetic research. Besides quantile normalization there is currently no standard method to normalize the data between arrays. We describe some properties of the data generated by this platform and present a normalization method based on local regression. We compare the performance of(More)
Long-lasting oxidative stress exposure may lead to relatively stable epigenetic modifications of the DNA in order to activate anti-oxidative defence mechanisms. Oxidative stress related DNA methylation may therefore be associated (causally or as a by-product) with cancer. We measured derivatives of reactive oxygen metabolites (D-ROM), total thiol levels(More)
BACKGROUND Timely diagnosis of cancer represents a challenging task; in particular, there is a need for reliable non-invasive screening tools that could achieve high levels of adherence at virtually no risk in population-based screening. In this review, we summarize the current evidence of exhaled breath analysis for cancer detection using standard analysis(More)
Every good clinician can distinguish type 1 (insulindependent) diabetes from type 2 (non-insulin dependent) diabetes, or at least they think they can. But the Boolean logic that underpins this traditional classification has recently been confounded by advances in genetics, immunology and biochemistry. Widely available laboratory tests have revealed the(More)
Colorectal cancer (CRC) is the third most common cancer worldwide. If detected at an early stage, prognosis is good. Despite increasing evidence for the benefits of implemented screening programs, such as screening colonoscopy, compliance is rather low. Hence there is demand for non-invasive tests for the early detection of CRC with high acceptance in(More)
AIM One concern in epigenome-wide studies investigating leukocyte DNA methylation is that observed associations may at least partly reflect differences in leukocyte composition (LC) rather than changes in methylation. We estimated the magnitude of confounding by LC for common risk factors and diseases. MATERIALS & METHODS Variation of LC according to sex,(More)
> library(normalize450K) > library(minfiData) ## this package includes some .idat files > library(data.table) > path <system.file("extdata",package="minfiData") > samples = fread(file.path(path, 'SampleSheet.csv'),integer64='character') > samples[,file:=file.path(path,Sentrix_ID,paste0(Sentrix_ID,'_',Sentrix_Position))] > ## samples$file is a character(More)
DNA methylation (DNAm) has been revealed to play a role in various diseases. Here we performed epigenome-wide screening and validation to identify mortality-related DNAm signatures in a general population-based cohort with up to 14 years follow-up. In the discovery panel in a case-cohort approach, 11,063 CpGs reach genome-wide significance (FDR<0.05). 58(More)
AIM Whole-blood DNA methylation depends on the underlying leukocyte composition and confounding hereby is a major concern in epigenome-wide association studies. Cell counts are often missing or may not be feasible. Computational approaches estimate leukocyte composition from DNA methylation based on reference datasets of purified leukocytes. We explored the(More)
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