Jon P. Chamberlain

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Tasks that demand externalized attention reliably suppress default network activity while activating the dorsal attention network. These networks have an intrinsic competitive relationship; activation of one suppresses activity of the other. Consequently, many assume that default network activity is suppressed during goal-directed cognition. We challenge(More)
Fluorography has recently achieved widespread usage for detection of radioactivity in polyacrylamide gels (1,2). Aqueous gels are equilibrated with dimethyl sulfoxide (DMSO),’ impregnated with fluor PPO dissolved in DMSO. soaked in water to remove DMSO, dried, and exposed to X-ray film at -70°C. The use of DMSO is necessitated by PPO’s insolubility in(More)
In order to investigate the regulation of actin gene transcription during early sea urchin development, a specific hybridization probe for actin sequences is required. Such a probe was produced by cloning cDNA transcribed from a sea urchin poly(A)-containing mRNA preparation enriched for actin message. Double-stranded DNA was ligated into the BamHI(More)
Sea urchin embryos contain a large cytoplasmic pool of microtubular protein, from which they can regenerate cilia several times without the need for synthesizing additional tubulin. The present studies were initiated to determine if a requirement for new tubulin synthesis can be induced by depleting this pool through repeated cycles of deciliation. By the(More)
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