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Architecture of a protein central to iron homeostasis: crystal structure and spectroscopic analysis of the ferric uptake regulator
TLDR
The first crystal structure of Fur from P. aeruginosa is presented and a model for the activation and DNA binding of the Fur protein is presented, using X‐ray absorption spectroscopic measurements and microPIXE analysis to characterize the distinct zinc and iron binding sites in solution.
A chromosomally encoded type III secretion pathway in Yersinia enterocolitica is important in virulence
TLDR
The ysaV mutant strain was somewhat attenuated in virulence compared with the wild type in the mouse oral model of infection (an approximately 0.9 log difference in LD50), and was nearly as virulent as the wildtype when inoculated intraperitoneally in the mice model.
Purification and Structural Characterization of Siderophore (Corynebactin) from Corynebacterium diphtheriae
TLDR
Mass spectrometry and NMR analysis demonstrated that corynebactin has a novel structure, consisting of a central lysine residue linked through its α- and ε- amino groups by amide bonds to the terminal carboxyl groups of two different citrate residues.
Development and use of a selectable, broad-host-range reporter transposon for identifying environmentally regulated promoters in bacteria.
TLDR
The development and use of TnKnXSp, a selectable broad-host-range reporter transposon with a promoterless aphA gene, are described and validated as a useful tool for identifying environmentally regulated promoters in bacteria.
A chromosomally encoded type III secretion pathway in Yersinia enterocolitica is important in virulence
TLDR
The ysaV mutant strain was somewhat attenuated in virulence compared with the wild type in the mouse oral model of infection (an approximately 0.9 log difference in LD50), and was nearly as virulent as the wildtype when inoculated intraperitoneally in the mice model.