Jolene R. Fredrickson

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OBJECTIVE To screen human serum albumin (HSA) preparations for toxicity and investigate causes of variation. DESIGN Experimental laboratory study. SETTING University-based laboratory. ANIMAL(S) FVB and CF1 mice crossed to create embryos used in experiments. INTERVENTION(S) Mouse embryo assay performed with 5% or 15% HSA (100 mg/mL albumin) from(More)
To determine the relative sensitivities of the 1 and 2-cell mouse embryo assays (MEA) and the human sperm motility assay (HSMA) for peroxides in mineral oil. The effect of peroxide on blastocyst cell number and apoptosis was also studied. One and two-cell MEA and HSMA were performed using mineral oil containing cumene hydroperoxide (CH). The 1-cell MEA was(More)
OBJECTIVE To determine the relative sensitivities of embryos from different strains of mice to in vitro stress. DESIGN Laboratory experiment with embryos from different mouse strains. SETTING University hospital-based fertility clinic. ANIMAL(S) Mice. INTERVENTION(S) Fresh one-cell embryos from outbred (CF1), inbred (FVB), F1 hybrid (B6/CBA), and(More)
To determine the composition of commercially available protein supplements for embryo culture media and test if differences in protein supplement composition are biologically relevant in a murine model. Amino acid, organic acid, ion and metal content were determined for 6 protein supplements: recombinant human albumin (AlbIX), human serum albumin (HSA and(More)
Controlled-rate frozen and vitrified mature human oocytes were thawed or warmed and parthenogenically activated using ionomycin and 6-dimethylaminopurine to assess pronuclear development compared with fresh controls. This chemical activation model provides a useful tool for laboratories to assess proficiency before offering oocyte cryopreservation to their(More)
Preimplantation genetic diagnosis for single gene disorders is usually performed using polymerase chain reaction (PCR)-based methodologies modified for use in single cells. At present, single cell PCR tests require costly and time-consuming development and validation of highly sensitive amplification strategies to cover a growing number of mutations(More)
The purpose of this study was to determine the effect of the protein stabilizer octanoic acid on blastocyst development, implantation, and fetal growth in a murine model. One-cell mouse embryos were collected and individually cultured in medium supplemented with recombinant human serum albumin for 96 h at 5 % oxygen in an EmbryoScope. Embryos were randomly(More)
OBJECTIVE To describe a unique case of recurrent aneuploidy and the use of preimplantation genetic screening (PGS). DESIGN Case report. SETTING Midwest academic medical center. PATIENT(S) A 36-year-old woman with two trisomy 21 offspring. INTERVENTION(S) Preimplantation genetic screening. MAIN OUTCOME MEASURE(S) Karyotype of embryos, liveborn(More)
HP1γ, a well-known regulator of gene expression, has been recently identified to be a target of Aurora A, a mitotic kinase which is important for both gametogenesis and embryogenesis. The purpose of this study was to define whether the Aurora A-HP1γ pathway supports cell division of gametes and/or early embryos, using western blot, immunofluorescence,(More)
Successful in vitro fertilization (IVF) relies on sound laboratory methods and culture conditions which depend on sensitive quality control (QC) testing. This study aimed to improve the sensitivity of mouse embryo assays (MEA) for detection of mineral oil toxicity. Five experiments were conducted to study modifications of the standard mouse embryo assay(More)
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