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PURPOSE To demonstrate a noninvasive method to visualize and analyze the parafoveal capillary network in humans. METHODS An adaptive optics scanning laser ophthalmoscope was used to acquire high resolution retinal videos on human subjects. Video processing tools that enhance motion contrast were developed and applied to the videos to generate montages of(More)
Articular cartilage function depends on the molecular composition and structure of its extracellular matrix (ECM). The collagen network (CN) provides cartilage with tensile integrity, but must also remodel during growth. Such remodeling may depend on matrix molecules interacting with the CN to modulate the tensile behavior of cartilage. The objective of(More)
Adaptive Optics Scanning Laser Ophthalmoscopy was used to noninvasively acquire videos of single-file flow through live human retinal parafoveal capillaries. Videos were analyzed offline to investigate capillary flow dynamics. Certain capillaries accounted for a clear majority of leukocyte traffic (Leukocyte-Preferred-Paths, LPPs), while other capillaries(More)
Microscopic features of the human retina can be resolved noninvasively using an adaptive optics scanning laser ophthalmoscope (AOSLO). We describe an improved method to track and quantify the speed of moving objects in AOSLO videos, which is necessary for characterizing the hemodynamics of retinal capillaries. During video acquisition, the objects of(More)
PURPOSE To establish, using adaptive optics scanning laser ophthalmoscopy (AOSLO), that the retinal parafoveal capillary network is altered before the onset of diabetic retinopathy in adult patients with type 2 diabetes. METHODS AOSLO videos were acquired in the parafoveal region of one eye from control subjects and from patients with type 2 diabetes and(More)
Multi-color stochastic optical reconstruction microscopy (STORM) is routinely performed; however, the various approaches for achieving multiple colors have important caveats. Color cross-talk, limited availability of spectrally distinct fluorophores with optimal brightness and duty cycle, incompatibility of imaging buffers for different fluorophores, and(More)
Recently, super-resolution microscopy methods such as stochastic optical reconstruction microscopy (STORM) have enabled visualization of subcellular structures below the optical resolution limit. Due to the poor temporal resolution, however, these methods have mostly been used to image fixed cells or dynamic processes that evolve on slow time-scales. In(More)
Stochastic optical reconstruction microscopy (STORM) and stimulated emission depletion (STED) microscopy are two super-resolution optical microscopy approaches that have rapidly gained popularity in recent years. Both modalities offer super-resolution imaging capabilities with the potential for imaging in multiple colors, three-dimensions, and the(More)
PURPOSE The purpose of this study was to establish that retinal pigment epithelial (RPE) cells take up indocyanine green (ICG) dye following systemic injection and that adaptive optics enhanced indocyanine green ophthalmoscopy (AO-ICG) enables direct visualization of the RPE mosaic in the living human eye. METHODS A customized adaptive optics scanning(More)
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