Johnny Tam

Guillaume Alan Cordier2
Melike Lakadamyali2
2Guillaume Alan Cordier
2Melike Lakadamyali
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Articular cartilage function depends on the molecular composition and structure of its extracellular matrix (ECM). The collagen network (CN) provides cartilage with tensile integrity, but must also remodel during growth. Such remodeling may depend on matrix molecules interacting with the CN to modulate the tensile behavior of cartilage. The objective of(More)
Adaptive Optics Scanning Laser Ophthalmoscopy was used to noninvasively acquire videos of single-file flow through live human retinal parafoveal capillaries. Videos were analyzed offline to investigate capillary flow dynamics. Certain capillaries accounted for a clear majority of leukocyte traffic (Leukocyte-Preferred-Paths, LPPs), while other capillaries(More)
Microscopic features of the human retina can be resolved noninvasively using an adaptive optics scanning laser ophthalmoscope (AOSLO). We describe an improved method to track and quantify the speed of moving objects in AOSLO videos, which is necessary for characterizing the hemodynamics of retinal capillaries. During video acquisition, the objects of(More)
  • Johnny Tam, Guillaume Alan Cordier, Joseph Steven Borbely, Ángel Sandoval Álvarez, Melike Lakadamyali
  • 2014
Multi-color stochastic optical reconstruction microscopy (STORM) is routinely performed; however, the various approaches for achieving multiple colors have important caveats. Color cross-talk, limited availability of spectrally distinct fluorophores with optimal brightness and duty cycle, incompatibility of imaging buffers for different fluorophores, and(More)
  • Johnny Tam, Guillaume Alan Cordier, Štefan Bálint, Ángel Sandoval Álvarez, Joseph Steven Borbely, Melike Lakadamyali
  • 2014
Recently, super-resolution microscopy methods such as stochastic optical reconstruction microscopy (STORM) have enabled visualization of subcellular structures below the optical resolution limit. Due to the poor temporal resolution, however, these methods have mostly been used to image fixed cells or dynamic processes that evolve on slow time-scales. In(More)
PURPOSE The purpose of this study was to establish that retinal pigment epithelial (RPE) cells take up indocyanine green (ICG) dye following systemic injection and that adaptive optics enhanced indocyanine green ophthalmoscopy (AO-ICG) enables direct visualization of the RPE mosaic in the living human eye. METHODS A customized adaptive optics scanning(More)
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