John W. Cosgrove

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A cell-free protein synthesis system, derived from brains of 3 mo-old male Fischer-344 rats, has been characterized. The optimum conditions for amino acid incorporation in the system were 5 mM magnesium ion and 200 mM potassium ion. Incorporation depended on the addition of ATP, GTP, and an energy-generating system, and was sensitive to addition of the(More)
A study of fold characteristics and deformation style using the evolution of the land surface: Zagros Simply Folded Belt, Iran" (2010). Fold-thrust belts form in the frontal regions of major contractional orogens, and are frequently important hydrocarbon provinces. Deformation within a fold-thrust belt varies both along and across its strike, on scales of(More)
Brains of male Fischer-344 rats aged 3-4 months and 28-30 months were dissected into 11 regions, and the patterns of brain proteins in these regions were analyzed using two dimensional gel electrophoresis in conjunction with a sensitive silver stain detection method. Several hundred abundant and moderately abundant brain proteins were detected in each(More)
A cell-free protein synthesis system was derived from brains of young (3 month) and old (greater than 23 month) male Fischer-344 rats in order to examine brain protein synthesis in relation to age. The system was shown to be capable of reinitiating protein synthesis in vitro, and of synthesizing protein from exogenously added mRNA. Optimal ionic conditions(More)
The slow hyperpolarizing afterpotential (slow HAP) following an evoked action potential in cultured rat muscle cells is inhibited by coculture with spinal cord neurons, spinal cord-conditioned medium (CM) and by a low-molecular-weight fraction (less than 4000 Da) prepared from this CM. The incidence, amplitude and duration of the slow HAPs decreased(More)
An initiating cell-free protein synthesis system derived from brain was utilized to demonstrate that the intravenous injection of D-lysergic acid diethylamide (LSD) to rabbits resulted in a lesion at the initiation stage of brain protein synthesis. Three inhibitors of initiation, edeine, poly(I), and aurintricarboxylic acid were used to demonstrate a(More)
Free and membrane-bound polysomes were isolated from the cerebral hemispheres and cerebellum of the young adult rabbit. The two polysomal populations were translated in an mRNA-dependent cell-free system derived from rabbit reticulocytes. Analysis of the [35S]methionine-labeled translation products on two-dimensional polyacrylamide gels indicated an(More)
Intravenous administration of LSD to young adult rabbits induces a transient disaggregation of brain polysomes and a relocalization of mRNA from polysomes to monosomes. To analyze the spectrum of mRNA molecules which were associated with either the residual polysomes or the translationally inactive monosome complex, these two fractions were isolated on(More)
Free and membrane-bound polysomes and polyadenylated mRNA isolated from rabbit brain were translated in an mRNA-dependent rabbit reticulocyte lysate system. Electrophoretic analysis of the cell-free translation products demonstrated that although most of the nascent proteins were common to both free and membrane-bound brain polysomes, qualitative and(More)