John T. Sexton

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Flow cytometry is widely used to measure gene expression and other molecular biological processes with single cell resolution via fluorescent probes. Flow cytometers output data in arbitrary units (a.u.) that vary with the probe, instrument, and settings. Arbitrary units can be converted to the calibrated unit molecules of equivalent fluorophore (MEF) using(More)
Living cells can be programmed with genetically encoded circuits to function as sophisticated decision-making devices, and communication among cells can facilitate increasingly complex, population-level computations. In this work, we describe a novel characterization platform for assessing the performance of genetically encoded circuits, and we demonstrate(More)
Chloroplast membranes of the green alga Chlamydomonas reinhardtii have been isolated from cells incubated in the presence of cycloheximide. The proteins produced under these conditions are formed on chloroplastic ribosomes. Fluorography of the polypeptides separated on polyacrylamide gels shows ten major protein species associated with the membranes that(More)
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