John Scullion

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This study aimed to evaluate metabolic fingerprinting by Fourier transform infrared (FT-IR) spectroscopy as a technique for investigating microbial communities and their activities in soil. FT-IR spectra from earthworm casts, and other 'biosamples', were compared using multivariate cluster analyses. The work formed part of a wider study to quantify the risk(More)
This study explores the utility of Fourier transform infra-red spectroscopy (FT-IR) as a metabolomic tool to detect changes in water-extractable chemical profile resulting from horizontal gene transfer (HGT) events in artificial soil slurries. A GFP–Km (Green fluorescent protein–kanamycin) cassette tagged HGT recipient Acinetobacter strain ADPWH67 with the(More)
We investigated whether exposure to UV-B results in biochemical changes in leaf litter, affecting growth rates of earth-worms feeding on this litter and cast chemistry. Seedlings of Betula pubescens L. were grown under zero and ambient UV-B (at 52 °N) regimes under optimal conditions. Following three months exposure, plants were allowed to senesce and leaf(More)
The complex and dynamic nature of soil makes the assessment of soil biodiversity difficult. Findings presented here form part of a wider scheme to develop 'bio-sampling' methods based on earthworm cast analysis. Our overall aim is to achieve high throughput, metabolic procedures to monitor soil biological activity and, in particular, to investigate the(More)
Rates of decomposition in Arctic soils are regulated by temperature and moisture, but substrate availability is dictated by vegetation inputs, which are also subject to biotic influences. Here, we examine how leaf and litter inputs from individual dwarf shrub species influence soil enzyme activity in a sub-Arctic heath community in Abisko, Sweden. We(More)
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