John S Jacobs Anderson

Learn More
Consensus pattern and matrix-based searches designed to predict cis-acting transcriptional regulatory sequences have historically been subject to large numbers of false positives. We sought to decrease false positives by incorporating expression profile data into a consensus pattern-based search method. We have systematically analyzed the expression(More)
APOBEC3F (A3F) and APOBEC3G (A3G) are DNA cytosine deaminases that potently restrict human immunodeficiency virus type 1 replication when the virus is deprived of its accessory protein Vif (virion infectivity factor). Vif counteracts these restriction factors by recruiting A3F and A3G to an E3 ubiquitin (Ub) ligase complex that mediates their(More)
The cell wall teichuronic acid (TUA) of Micrococcus luteus is a long-chain polysaccharide composed of disaccharide repeating units [-4-β-D-ManNAcAp-(1→6)α-D-Glcp-1-](n), which is covalently anchored to the peptidoglycan on the inner cell wall and extended to the outer surface of the cell envelope. An enzyme complex responsible for the TUA chain biosynthesis(More)
APOBEC3 family DNA cytosine deaminases provide overlapping defenses against pathogen infections. However, most viruses have elaborate evasion mechanisms such as the HIV-1 Vif protein, which subverts cellular CBF-β and a polyubiquitin ligase complex to neutralize these enzymes. Despite advances in APOBEC3 and Vif biology, a full understanding of this direct(More)
The process of RNA turnover is important both for the control of mRNA levels and for the degradation of excised introns. In several cases, the degradation of transcripts is now known to involve exonucleolytic digestion of the RNA, either directly, or following an initiating nucleolytic event, such as decapping (the removal of the structure that(More)
The in vitro biosynthesis of teichuronic acid, the cell wall polysaccharide of Micrococcus lysodeikticus, occurs in two stages. In the initial stage, the particulate enzyme fraction utilizes uridine diphosphate N-acetylglucosamine (UDPGlcNAc) and uridine diphosphate N-acetylmannosaminuronic acid (UDP-ManNAcUA) to form three intermediates.(More)
The particulate enzyme fraction obtained from Micrococcus Zysodeikficus catalyzed the incorporation of D-[r4C]glucose from uridine diphospho-n-[r4C]glucose into a polymer which resembled the n-glucoseand N-acetyl-n-mannosaminuronic acid-containing polysaccharide of the cell walls of this organism. In vifro biosynthesis of the polysaccharide depended upon(More)
The homologous proteins from the egg whites of different avian species offer excellent opportunities for the study of the comparative and genetic biochemistry of proteins. Comparative studies of avian egg whites have utilized electrophoretic (l-4), immunological (5, 6), and specific biochemical (7, 8) analyses. These studies have all shown significant, and(More)
  • 1