John R. Lacey

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This study investigated the environmental conditions on pig farms and the respiratory health of pig farmers and their immunological response to airborne contaminants. Airborne concentrations of dust and ammonia were measured in 20 pig houses; viable microorganisms, endotoxins, and aeroallergens were measured in 6 of these houses, chosen to represent the(More)
After several cases of occupational asthma had been reported in a coffee processing factory in England, 197 coffee workers representing 80% of the production workforce were studied to determine the factors affecting the development of work related respiratory symptoms of wheeze, cough, and dyspnoea. Two computer administered questionnaires concerning the(More)
Maps were made of the distributions of tubers of plants of 15 potato varieties growing in the field. Although distribution was mainly a varietal characteristic, there was some evidence that it could be affected by soil type and seasonal differences. Varieties with short stolons (e.g.Ulster Tarn) not only had tubers tightly clustered, but tended to have many(More)
A novel containment system using a microporous film material is described for laboratory-scale solid particulate fermentations. The microporous film allows efficient air and moisture vapour transmission without cross-contamination. The non-autoclaved substrates support vigorous fungal growth and sporulation within the containment bags.
Scanning electron microscopy (SEM), light microscopy (LM), epifluorescence microscopy (FM), and culture were used to assess catches of microorganisms in parallel air samples on membrane filters from heavily contaminated working environments that differed in the relative abundance of bacteria, actinomycetes, and fungal spores. Except in pig houses, estimates(More)
Projects funded by International Development Research Centre (IDRC) of Canada and the European Commission have enabled the examination of more than 3000 samples of maize collected from all regions of Costa Rica at different stages, from the growing crop through storage to final sale, and at different water contents. Contamination with Aspergillus flavus was(More)