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Polyamines have been shown to play a role in the growth and survival of several solid tumors, including colorectal cancer. We identified the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase (SSAT) as being one of the most highly inducible genes in two DNA microarray screens to identify novel determinants of response to chemotherapeutic(More)
DNA microarray analysis was used to analyze the transcriptional profile of HCT116 colorectal cancer cells that were treated with 5-fluorouracil (5-FU) or oxaliplatin and selected for resistance to these agents. Bioinformatic analyses identified sets of genes that were constitutively dysregulated in drug-resistant cells and transiently altered following(More)
A graph is planar if it can be drawn on the plane with no crossing edges. There are several linear time planar embedding algorithms but all are considered by many to be quite complicated. This paper presents a new method for performing linear time planar graph embedding which avoids some of the complexities of previous approaches (including the need to rst(More)
As the world wide web transforms from a vehicle of information dissemination and e-commerce transactions into a writable nexus of human collaboration, the Web 2.0 technologies at the forefront of the tranformation may be seen as special cases of a more general shift in the conceptual application model of the web. This paper recognizes the conceptual(More)
The standard unit of work in the business process is the electronic form, which includes complex user interface designs, data gathering and validation, wizard-like behaviors and spreadsheet computations. This paper reports state-of-the-art digital signature methods used to help provide security, non-repudiation and auditability within complex electronic(More)
We present a simple pedagogical graph theoretical description of Lempel, Even, and Cederbaum (LEC) planarity method based on concepts due to Thomas. A linear-time implementation of LEC method using the PC-tree data structure of Shih and Hsu is provided and described in details. We report on an experimental study involving this implementation and other(More)
The fluoropyrimidine 5-Fluorouracil (5-FU) is widely used in the treatment of cancer. To identify novel downstream mediators of tumor cell response to 5-FU, we used DNA microarray technology to identify genes that are transcriptionally activated by 5-FU treatment in the MCF-7 breast cancer cell line. Of 2400 genes analyzed, 619 were up-regulated by >3-fold.(More)
To elucidate mechanisms of resistance to chemotherapies currently used in the first-line treatment of advanced colorectal cancer, we have developed a panel of HCT116 p53 wild-type (p53(+/+)) and null (p53(-/-)) isogenic colorectal cancer cell lines resistant to the antimetabolite 5-fluorouracil (5-FU), topoisomerase I inhibitor irinotecan (CPT-11), and(More)
Thymidylate synthase (TS) is a critical target for chemotherapeutic agents such as 5-fluorouracil (5-FU) and antifolates such as tomudex (TDX),multitargeted antifolate, and ZD9331. Using the MCF-7 breast cancer line, we have developed p53 wild-type (M7TS90) and null (M7TS90-E6) isogenic lines with inducible TS expression (approximately 6-fold induction(More)
Fas (CD95/Apo-1) is a member of the tumor necrosis factor receptor family. Receptor binding results in activation of caspase 8, leading to activation of proapoptotic downstream molecules. We found that expression of Fas was up-regulated >10-fold in MCF-7 breast and HCT116 and RKO colon cancer cell lines after treatment with IC(60) doses of 5-fluorouracil(More)