John J. Love

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Designing proteins with novel protein/protein binding properties can be achieved by combining the tools that have been developed independently for protein docking and protein design. We describe here the sequence-independent generation of protein dimer orientations by protein docking for use as scaffolds in protein sequence design algorithms. To dock(More)
Lymphoid enhancer-binding factor-1 (LEF-1), a member of the high-mobility group (HMG) family of proteins, functions as an architectural transcription factor. In complex with its cognate DNA, the LEF-1 domain is highly ordered, and its NMR spectra are characteristic of a folded globular protein. In contrast, the uncomplexed protein exhibits NMR evidence of(More)
Protein fibril formation is implicated in many diseases, and therefore much effort has been focused toward the development of inhibitors of this process. In a previous project, a monomeric protein was computationally engineered to bind itself and form a heterodimer complex following interfacial redesign. One of the protein monomers, termed monomer-B, was(More)
The correlation between protein structure and function is well established, yet the role stability/flexibility plays in protein function is being explored. Here, we describe an in vivo screen in which the thermal stability of a test protein is correlated directly to the transcriptional regulation of a reporter gene. The screen readout is independent of the(More)
Polyamides composed of N-methylpyrrole (Py), N-methylimidazole (Im) and N-methylhydroxypyrrole (Hp) amino acids linked by beta-alanine (beta) bind the minor groove of DNA in 1:1 and 2:1 ligand to DNA stoichiometries. Although the energetics and structure of the 2:1 complex has been explored extensively, there is remarkably less understood about 1:1(More)
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