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Crystal Structures of a Complexed and Peptide-Free Membrane Protein–Binding Domain: Molecular Basis of Peptide Recognition by PDZ
TLDR
X-ray crystallographic structures of the third PDZ domain from the synaptic protein PSD-95 in complex with and in the absence of its peptide ligand have been determined and reveal that specific side chain interactions and a prominent hydrophobic pocket explain the selective recognition of the C-terminal consensus sequence. Expand
Spatial Localization of the K+ Channel Selectivity Filter by Mutant Cycle–Based Structure Analysis
TLDR
This study constrains the position of multiple channel residues within the pore-forming loops; in one stretch, it is able to map five out of seven contiguous residues to the inhibitor interaction surface, including those involved in ion selectivity. Expand
Myosin I Can Act As a Molecular Force Sensor
TLDR
The ability to sense molecular tension is crucial for a wide array of cellular processes, including the detection of auditory stimuli, control of cell shape, and internalization and transport of membranes, and a role for myosin I as a molecular force sensor is supported. Expand
Temperature dependence of nucleotide association and kinetic characterization of myo1b.
TLDR
The rates and temperature dependence of ADP release are similar to k(alpha) suggesting that a similar structural change is responsible for both transitions, and a duty ratio of 0.08 is calculated based on the biochemical kinetics. Expand
Calcium regulation of myosin-I tension sensing.
TLDR
Calcium accelerates key biochemical transitions on the ATPase pathway, decreases the working-stroke displacement, and greatly reduces the ability of myo1b to sense tension, which provides an effective mechanism for inhibiting motility and terminating long-duration attachments. Expand
The azimuthal path of myosin V and its dependence on lever-arm length
TLDR
P polarized total internal reflection fluorescence microscopy is used to examine angular changes of bifunctional rhodamine probes on the lever arms of single myoV molecules in vitro and shows that the azimuthal deflections of myosin on actin are more common for the truncated lever arm, but the range of these deflections is relatively independent of its lever-arm length. Expand
Control of myosin-I force sensing by alternative splicing
TLDR
This study examined force sensing by the widely expressed myosin-I isoform, myo1b, which is alternatively spliced in its light chain binding domain (LCBD), yielding proteins with lever arms of different lengths, and found the actin-detachment kinetics of the splice isoforms to be extraordinarily tension-sensitive. Expand
Calcium regulation of calmodulin binding to and dissociation from the myo1c regulatory domain.
TLDR
The data suggest that the calmodulin bound to the IQ motif adjacent to the motor domain is rapidly exchangeable in the presence of calcium and is responsible for regulation of myo1c ATPase and motile activity. Expand
MEMLET: An Easy-to-Use Tool for Data Fitting and Model Comparison Using Maximum-Likelihood Estimation.
TLDR
A comparison of MLE and other fitting techniques is given, showing how MLE often outperforms other fitting methods, and packages all of these techniques into a simple-to-use and well-documented interface to increase the accessibility of Mle fitting. Expand
Accessible, Feature-Rich Software for Rigorous Model Fitting using Maximum Likelihood Estimation
TLDR
This presentation includes a comparison of MLE to other fitting techniques that utilize binning, cumulative frequency distributions, etc., showing that MLE often outperforms other commonly used fitting methods. Expand
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